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http://hdl.handle.net/10261/308230
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Caprini, Marco | es_ES |
dc.contributor.author | Gomis, Ana | es_ES |
dc.contributor.author | Cabedo, Hugo | es_ES |
dc.contributor.author | Planells-Cases, Rosa | es_ES |
dc.contributor.author | Belmonte, Carlos | es_ES |
dc.contributor.author | Viana, Félix | es_ES |
dc.contributor.author | Ferrer-Montiel, Antonio | es_ES |
dc.date.accessioned | 2023-05-04T12:36:22Z | - |
dc.date.available | 2023-05-04T12:36:22Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | EMBO Journal 22(12): 3004-3014 (2003) | es_ES |
dc.identifier.issn | 0261-4189 | - |
dc.identifier.uri | http://hdl.handle.net/10261/308230 | - |
dc.description.abstract | The identification of osmo/mechanosensory proteins in mammalian sensory neurons is still elusive. We have used an expression cloning approach to screen a human dorsal root ganglion cDNA library to look for proteins that respond to hypotonicity by raising the intracellular Ca2+ concentration ([Ca2+]i). We report the unexpected identification of GAP43 (also known as neuromodulin or B50), a membrane-anchored neuronal protein implicated in axonal growth and synaptic plasticity, as an osmosensory protein that augments [Ca2+]i in response to hypotonicity. Palmitoylation of GAP43 plays an important role in the protein osmosensitivity. Depletion of intracellular stores or inhibition of phospholipase C (PLC) activity abrogates hypotonicity-evoked, GAP43-mediated [Ca2+]i elevations. Notably, hypotonicity promoted the selective association of GAP43 with the PLC-δ1 isoform, and a concomitant increase in inositol-1,4,5-trisphosphate (IP3) formation. Collectively, these findings indicate that hypo-osmotic activation of GAP43 induces Ca2+ release from IP3-sensitive intracellular stores. The osmosensitivity of GAP43 furnishes a mechanistic framework that links axon elongation with phospho inositide metabolism, spontaneous triggering of cytosolic Ca2+ transients and the regulation of actin dynamics and motility at the growth cone in response to temporal and local mechanical forces. | es_ES |
dc.description.sponsorship | A.G. was a fellow of the CSIC-IP3 program from European Social Funds, and M.C. was a fellow of the Marie Curie Host Fellowship Program. This work was supported by grants from La Fundación La Caixa (01/085-00 to A.F.-M.), the Spanish Ministry of Science and Technology (MCYT) (SAF2000-0142 to A.F.-M., SAF2001-1641 to F.V. and BFI2002-03788 to C.B.), the Instituto de la Salud Carlos III (FIS-01/1162 to C.B.) and The Generalitat Valenciana (GV01-01 to A.F.-M). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | EMBO Press | es_ES |
dc.rights | closedAccess | es_ES |
dc.title | GAP43 stimulates inositol trisphosphate-mediated calcium release in response to hypotonicity | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1093/emboj/cdg294 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1093/emboj/cdg294 | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Fundación la Caixa | es_ES |
dc.contributor.funder | Ministerio de Ciencia y Tecnología (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003359 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100006280 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.pmid | 12805215 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
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