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http://hdl.handle.net/10261/307086
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Ortiz-Padilla, Miriam | es_ES |
dc.contributor.author | Portillo-Calderón, Inés | es_ES |
dc.contributor.author | Maldonado, Natalia | es_ES |
dc.contributor.author | Rodríguez-Martínez, José-Manuel | es_ES |
dc.contributor.author | Gregorio-Iaria, Belén de | es_ES |
dc.contributor.author | Merino-Bohórquez, Vicente | es_ES |
dc.contributor.author | Rodríguez-Baño, Jesús | es_ES |
dc.contributor.author | Pascual, Álvaro | es_ES |
dc.contributor.author | Docobo-Pérez, Fernando | es_ES |
dc.date.accessioned | 2023-04-21T10:04:32Z | - |
dc.date.available | 2023-04-21T10:04:32Z | - |
dc.date.issued | 2022-02 | - |
dc.identifier.citation | Clinical Microbiology and Infection 28(2): 302.e1-302.e4 (2022) | es_ES |
dc.identifier.issn | 1198-743X | - |
dc.identifier.uri | http://hdl.handle.net/10261/307086 | - |
dc.description.abstract | [Objectives] The objective of this study was to evaluate the in vitro activity of fosfomycin under different physiological concentrations of inorganic phosphate (Pi). | es_ES |
dc.description.abstract | [Methods] The wild-type BW25113 strain, four isogenic mutants (ΔglpT, ΔuhpT, ΔglpT-uhpT, and ΔphoB) and six clinical isolates of Escherichia coli with different fosfomycin susceptibilities were used. EUCAST breakpoints were used. Susceptibility was evaluated by agar dilution using standard Mueller–Hinton agar (Pi concentration of 1 mM similar to human plasma concentration) and supplemented with Pi (13 and 42 mM, minimum and maximum urinary Pi concentrations) and/or glucose-6-phosphate (25 mg/L). Fosfomycin transporter promoter activity was assayed using PglpT::gfpmut2 or PuhpT::gfpmut2 promoter fusions in standard Mueller–Hinton Broth (MHB), supplemented with Pi (13 or 42 mM) ± glucose-6-phosphate. Fosfomycin activity was quantified, estimating fosfomycin EC50 under different Pi concentrations (1, 13 and 42 mM + glucose-6-phosphate) and in time–kill assays using fosfomycin concentrations of 307 (maximum plasma concentration (Cmax)), 1053 and 4415 mg/L (urine Cmax range), using MHB with 28 mM Pi (mean urine Pi concentration) + 25 mg/L glucose-6-phosphate. | es_ES |
dc.description.abstract | [Results] All the strains showed decreased susceptibility to fosfomycin linked to increased Pi concentrations: 1–4 log2 dilution differences from 1 to 13 mM, and 1–8 log2 dilution differences at 42 mM Pi. Changes in phosphate concentration did not affect the expression of fosfomycin transporters. By increasing Pi concentrations higher fosfomycin EC50 bacterial viability was observed, except against ΔglpT-uhpT. The increase in Pi reduced the bactericidal effect of fosfomycin. | es_ES |
dc.description.abstract | [Discussion] Pi variations in physiological fluids may reduce fosfomycin activity against E. coli. Elevated Pi concentrations in urine may explain oral fosfomycin failure in non-wild-type but fosfomycin-susceptible E. coli strains. | es_ES |
dc.description.sponsorship | This study has been funded by Instituto de Salud Carlos III through the projects PI16/01824, PI19/01645 and REIPI RD16/0016/0001 (co-funded by European Regional Development Fund “A way to make Europe”). Miriam Ortiz-Padilla is supported by a PFIS grant from the Instituto de Salud Carlos III (FI17/00235). Inés Portillo-Calderón is supported by a Rio Hortega grant from the Instituto de Salud Carlos III (CM20/0092). (Co-funded by European Social Fund “Investing in your future”). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO//RD16%2F0016%2F0001/ES/RED ESPAÑOLA DE INVESTIGACIÓN EN PATOLOGÍAS INFECCIOSAS/ | es_ES |
dc.relation | info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII)/PI19%2F01645/ES/EFECTO DE LOS TRANSPORTADORES EN LA ACTIVIDAD DE FOSFOMICINA IN VITRO E IN VIVO: OPTIMIZACION DEL ENSAYO DE SENSIBILIDAD IN VITRO Y CARACTERIZACION DE POTENCIALES ACTIVADORES DE LA EFICACIA IN VIVO./ | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO//PI16%2F01824/ES/Caracterización de la resistencia y heterorresistencia a fosfomicina en enterobacterias. Optimización del potencial terapéutico de fosfomicina frente a enterobacterias multirresistentes/ | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Resistance | es_ES |
dc.subject | Escherichia coli | es_ES |
dc.subject | Fosfomycin | es_ES |
dc.subject | Fosfomycin activity | es_ES |
dc.subject | Fosfomycin transporters | es_ES |
dc.subject | Inorganic phosphates | es_ES |
dc.title | Role of inorganic phosphate concentrations in in vitro activity of fosfomycin | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.cmi.2021.09.037 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.cmi.2021.09.037 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Red Española de Investigación en Patología Infecciosa | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.pmid | 34634457 | - |
dc.identifier.scopus | 2-s2.0-85118255617 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85118255617 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.openairetype | artículo | - |
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