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Título: | Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression |
Autor: | Hernández-Ortego, Pablo CSIC ORCID; Torres-Montero, Remedios CSIC; Peña, Elvira de la CSIC ORCID; Viana, Félix CSIC ORCID ; Fernández-Trillo, Jorge CSIC ORCID | Palabras clave: | TRPM8 Western blot Immunocytochemistry Immunohistochemistry |
Fecha de publicación: | 18-dic-2022 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | International Journal of Molecular Sciences 23(24): 16164 (2022) | Resumen: | TRPM8 is a non-selective cation channel expressed in primary sensory neurons and other tissues, including the prostate and urothelium. Its participation in different physiological and pathological processes such as thermoregulation, pain, itch, inflammation and cancer has been widely described, making it a promising target for therapeutic approaches. The detection and quantification of TRPM8 seems crucial for advancing the knowledge of the mechanisms underlying its role in these pathophysiological conditions. Antibody-based techniques are commonly used for protein detection and quantification, although their performance with many ion channels, including TRPM8, is suboptimal. Thus, the search for reliable antibodies is of utmost importance. In this study, we characterized the performance of six TRPM8 commercial antibodies in three immunodetection techniques: Western blot, immunocytochemistry and immunohistochemistry. Different outcomes were obtained for the tested antibodies; two of them proved to be successful in detecting TRPM8 in the three approaches while, in the conditions tested, the other four were acceptable only for specific techniques. Considering our results, we offer some insight into the usefulness of these antibodies for the detection of TRPM8 depending on the methodology of choice. | Descripción: | This article belongs to the Special Issue Targeting TRP Channels for Pain, Itch and Inflammation Relief. | Versión del editor: | https://doi.org/10.3390/ijms232416164 | URI: | http://hdl.handle.net/10261/304491 | DOI: | 10.3390/ijms232416164 | ISSN: | 1661-6596 |
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TRPM8 Ab validation Hernandez_Ortego Viana IJMS 2022.pdf | Articulo | 4,34 MB | Adobe PDF | Visualizar/Abrir |
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