Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/294462
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A strain-type clustering of potato virus Y based on the genetic distance between isolates calculated by RFLP analysis of the amplified coat protein gene |
Autor: | Blanco-Urgoiti, B.; Sánchez, Flora; Dopazo, J.; Ponz Ascaso, Fernando | Fecha de publicación: | 1996 | Editor: | Springer Nature | Citación: | Archives of Virology 141: 2425-2442 (1996) | Resumen: | Potato virus Y (PVY) isolates have been classified into genetic strains by a host-independent criterion using a molecular typing method. The method used extracts from infected tissue, and included immunocapture-RT-PCR-RFLP analysis using 5 different restriction endonucleases (Dde I, Eco RV, Hinf I, Rsa I and Taq I). Genetic distances between the different PVY 'restrictotypes' were calculated and used to define the PVY genetic strains. Three main clusters were found PVY(O), PVY(N), and non-potato PVY (PVY(NP)) in good agreement with classical PVY strain definitions that combine different biological criteria. Our approach was incomparably quicker and more reliable and reproducible than biotyping. The potential of this approach for very quick, simple and automatable molecular epidemiological studies is discussed. | URI: | http://hdl.handle.net/10261/294462 | DOI: | 10.1007/BF01718641 | ISSN: | 0304-8608 | E-ISSN: | 1432-8798 |
Aparece en las colecciones: | (INIA) Artículos |
Mostrar el registro completo
CORE Recommender
SCOPUSTM
Citations
36
checked on 11-may-2024
WEB OF SCIENCETM
Citations
36
checked on 29-feb-2024
Page view(s)
14
checked on 16-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.