An effect of various nitrogen forms used as fertilizer on Lupinus albus L. yield and protein, alkaloid and α-galactosides content

Abstract

The influence of various forms of nitrogen supplied to the ground as N 2, NH4+, NO3-, [NH 4+ + NO3-], -NH2, and Nd (N-deficiency) on Lupinus albus L. cvs. Butan (sweet) and Bac (bitter) yield and protein, alkaloid and α-galactoside biosynthesis in their seeds were studied. The experiments were performed in a greenhouse on perlite using, in all cases, the same dose of P, K, Mg and micronutrients (B, Zn. Mn, Cu, Mo, Fe). The Nd treatment contained only macro- and micronutrients without any nitrogen support. We found that different nitrogen forms used as a fertilizer had a significant influence on the development and yield structure of both lupin cultivars. It was found that the plants developed most favourably when nitrogen was supplied as N2 and [NH4+ + NO 3-]. For the other forms, the anomalies like necrosis, chlorosis, leaves with small surface of assimilation were noticed. However, these observations were not always reflected by all parameters of yield structure. The forms of nitrogen had a pivotal influence on yield of generative and vegetative parts of lupin. Moreover, significant differences in protein, alkaloid and α-galactoside content in seeds were observed. © 2005 Blackwell Verlag.