Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/292389
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | PCR amplification of the gene acmA differentiates Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris |
Autor: | Garde López-Brea, Sonia; Babín, M. del Mar; Gaya Sicilia, María Pilar; Núñez Gutiérrez, Manuel; Medina, Milagros CSIC ORCID | Fecha de publicación: | 1999 | Editor: | American Society for Microbiology | Citación: | Applied and Environmental Microbiology 65(11): 5151-5153 (1999) | Resumen: | The occurrence of the acmA gene, encoding the lactococcal N- acetylmuramidase in new lactococcal isolates from raw milk cheeses, has been determined. Isolates were genotypically identified to the subspecies level with a PCR technique. On the basis of PCR amplification of the acmA gene, the presence or absence of an additional amplicon of approximately 700 bp correlated with Lactococcus lactis subspecies. L. lactis subsp. lactis exhibits both the expected 1,131-bp product and the additional amplicon, whereas L. lactis subsp. cremoris exhibits a single 1,131-bp fragment. | URI: | http://hdl.handle.net/10261/292389 | ISSN: | 0099-2240 | E-ISSN: | 1098-5336 |
Aparece en las colecciones: | (INIA) Artículos |
Mostrar el registro completo
CORE Recommender
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.