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Título: | Characterization of natural ejaculates and sperm cryopreservation in a golden eagle (Aquila chrysaetus) |
Autor: | Villaverde-Morcillo, S.; García-Sánchez, Rubén; Castaño, Cristina; Rodríguez-Romero, Eduar; González, Fernando; Esteso, Milagros C. CSIC ORCID; Santiago Moreno, Julián | Palabras clave: | Aquila chrysaetus Cryopreservation Ejaculate Golden eagle Semen |Spermatozoa |
Fecha de publicación: | 2015 | Editor: | American Association of Zoo Veterinarians | Citación: | Journal of Zoo and Wildlife Medicine 46(2): 335-338 (2015) | Resumen: | This paper describes the sperm characteristics and response to cooling and freezing of naturally ejaculated semen from a captive, adult golden eagle (Aquila chrysaetus) trained to allow sperm recovery via cooperative copulation. A basic spermiogram was prepared, and sperm motility and morphometric variables recorded using a computer-aided system. For sperm storage, the effects of a polyvinylpyrrolidone-based extender were evaluated at 5°C. The same extender was also used in freezing procedures in which glycerol (11%) and dimethylacetamide (6%) were compared as cryoprotectants. The extender preserved sperm viability over storage periods of up to 6 days. Although sperm motility and percentage live sperm values were poorer for frozen-thawed (5.8-14.6% and 44-42%, respectively) than for fresh samples (46.7 and 74.6%, respectively), no differences were seen between the effects of the two cryoprotectants. These results could be of use when attempting to store the sperm of golden eagles and other raptors. © 2015 American Association of Zoo Veterinarians. | URI: | http://hdl.handle.net/10261/290044 | DOI: | 10.1638/2013-0293R1.1 | ISSN: | 1042-7260 | E-ISSN: | 1937-2825 |
Aparece en las colecciones: | (INIA) Artículos |
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