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Título: | Neutralization of viral infectivity by zebrafish c-reactive protein isoforms |
Autor: | Belló Pérez, Melissa; Falcó, Alberto; Medina-Gali, Regla María; Pereiro, Patricia CSIC ORCID; Encinar, José Antonio; Novoa, Beatriz CSIC ORCID; Pérez, L.; Coll Morales, Julio | Palabras clave: | C-reactive protein Zebrafish CRP | | Microarrays Anti-viral neutralizing activity VHSV SVCV |
Fecha de publicación: | 2017 | Editor: | Elsevier | Citación: | Molecular Immunology 91: 145-155 (2017) | Resumen: | This work explores the unexpected in vivo and in vitro anti-viral functions of the seven c-reactive protein (crp1-7) genes of zebrafish (Danio rerio). First results showed heterogeneous crp1-7 transcript levels in healthy wild-type zebrafish tissues and organs and how those levels heterogeneously changed not only after bacterial but also after viral infections, including those in adaptive immunity-deficient rag1-/- mutants. As shown by microarray hybridization and proteomic techniques, crp2/CRP2 and crp5/CRP5 transcripts/proteins were among the most modulated during in vivo viral infection situations including the highest responses in the absence of adaptive immunity. In contrast crp1/CRP1/and crp7/CRP7 very often remained unmodulated. All evidences suggested that zebrafish crp2-6/CRP2-6 may have in vivo anti-viral activities in addition to their well known anti-bacterial and/or physiological functions in mammalians. Confirming those expectations, in vitro neutralization and in vivo protection against spring viremia carp virus (SVCV) infections were demonstrated by crp2-6/CRP2-6 using crp1-7 transfected and/or CRP1-7-enriched supernatant-treated fish cells and crp2-5-injected one-cell stage embryo eggs, respectively. All these findings discovered a crp1-7/CRP1-7 primitive anti-viral functional diversity.These findings may help to study similar functions on the one-gene-coded human CRP, which is widely used as a clinical biomarker for bacterial infections, tissue inflammation and coronary heart diseases. | URI: | http://hdl.handle.net/10261/289571 | DOI: | 10.1016/j.molimm.2017.09.005 | ISSN: | 0161-5890 |
Aparece en las colecciones: | (INIA) Artículos (IIM) Artículos |
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