Spatial isolation impacts pollinator visitation and reproductive success of a threatened self-incompatible Mediterranean tree

Abstract

Pollination service is crucial to achieve successful plant sexual reproduction and long-term population persistence. This pollination service can be affected by plant conspecific density and also by intrinsic features of individuals related to their flowering phenology and floral display. However, studies examining intrinsic and extrinsic traits on pollinator visitation and reproductive success of Mediterranean trees with limited reproduction are still scarce. We analyzed the effects of plant isolation, flowering phenology, flower weight and tree size on flower visitation probability, flowering patch visitation probability, fruit set and crop size. To this end, we intensively monitored pollinator visitation and fruit production of 67 (in 2019) and 73 (in 2020) Pyrus bourgaeana Decne trees within a threatened Mediterranean population. Our results revealed that isolated individuals received more pollinators than those on conspecific aggregations, suggesting intraspecific competition for pollinators in dense flowering neighborhoods. However, fruit set was higher in trees close to flowering conspecifics despite having fewer visits from pollinators, suggesting pollen limitation but not pollinator limitation in spatially isolated trees. Interestingly, we found increased crop sizes in spatially isolated trees which could be related to reduced intraspecific competition for resources in low-density neighborhoods (water, nutrients) and/or to higher reproductive investment (i.e. higher flower production). Overall, our results indicated pollen but not pollinator limitation in spatially isolated trees. Under this scenario of sexual reproduction mediated by pollinators, our findings stress the relevance of individuals' spatial distribution for self-incompatible trees exhibiting low individuals’ densities.