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| Título: | Using redox-sensitive fluorescent probes to record real-time reactive oxygen species production in cells from mouse carotid body slices |
Autor: | Gao, Lin CSIC ORCID; Arias-Mayenco, Ignacio CSIC ORCID; Ortega-Sáenz, Patricia CSIC ORCID CVN; López-Barneo, José CSIC ORCID | Palabras clave: | Cell biology Metabolism Microscopy Molecular/Chemical Probes |
Fecha de publicación: | 18-jun-2021 | Editor: | Cell Press | Citación: | STAR Protocols 2(2): 100535 (2021) | Resumen: | Reactive oxygen species (ROS) are important signaling molecules for physiologic processes such as acute response to hypoxia. However, reliable real-time ROS measurement in cells has been a long-standing methodological challenge. Here, we present a protocol to record acute changes in ROS production in sensory cells from mouse carotid body (CB) slices using redox-sensitive green fluorescent protein probes and microfluorimetry. This protocol provides sensitive and reproducible quantification of ROS during acute hypoxia in different subcellular compartments of CB glomus cells. | Descripción: | For complete details on the use and execution of this protocol, please refer to Fernández-Agüera et al. (2015) (10.1016/j.cmet.2015.09.004) and Arias-Mayenco et al. (2018) (10.1016/j.cmet.2018.05.009). | Versión del editor: | http://dx.doi.org/10.1016/j.xpro.2021.100535 | URI: | http://hdl.handle.net/10261/266637 | DOI: | 10.1016/j.xpro.2021.100535 | Identificadores: | doi: 10.1016/j.xpro.2021.100535 e-issn: 2666-1667 |
| Aparece en las colecciones: | (IBIS) Artículos |
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| mouse_carotid_body.pdf | 2,87 MB | Adobe PDF |  Visualizar/Abrir |
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