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Título: | Inhibition of APE1/Ref-1 Redox Signaling Alleviates Intestinal Dysfunction and Damage to Myenteric Neurons in a Mouse Model of Spontaneous Chronic Colitis |
Autor: | Sahakian, L.; Filippone, R. T.; Stavely, R.; Robinson, A. M.; Yan, X. S.; Abalo, Raquel CSIC ORCID; Eri, R.; Bornstein, J. C.; Kelley, M. R.; Nurgali, Kulmira | Palabras clave: | APE1/Ref-1 APX3330 enteric nervous system chronic intestinal inflammation IBD, oxidative stress DNA damage |
Fecha de publicación: | 2021 | Citación: | Inflammatory Bowel Diseases 27: 388-406 (2021) | Resumen: | Background: Inflammatory bowel disease (IBD) associates with damage to the enteric nervous system (ENS), leading to gastrointestinal (GI) dysfunction. Oxidative stress is important for the pathophysiology of inflammation-induced enteric neuropathy and GI dysfunction. Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a dual functioning protein that is an essential regulator of the cellular response to oxidative stress. In this study, we aimed to determine whether an APE1/Ref-1 redox domain inhibitor, APX3330, alleviates inflammation-induced oxidative stress that leads to enteric neuropathy in the Winnie murine model of spontaneous chronic colitis. Methods: Winnie mice received APX3330 or vehicle via intraperitoneal injections over 2 weeks and were compared with C57BL/6 controls. In vivo disease activity and GI transit were evaluated. Ex vivo experiments were performed to assess functional parameters of colonic motility, immune cell infiltration, and changes to the ENS. Results: Targeting APE1/Ref-1 redox activity with APX3330 improved disease severity, reduced immune cell infiltration, restored GI function, and provided neuroprotective effects to the enteric nervous system. Inhibition of APE1/Ref-1 redox signaling leading to reduced mitochondrial superoxide production, oxidative DNA damage, and translocation of high mobility group box 1 protein (HMGB1) was involved in neuroprotective effects of APX3330 in enteric neurons. Conclusions: This study is the first to investigate inhibition of APE1/Ref-1's redox activity via APX3330 in an animal model of chronic intestinal inflammation. Inhibition of the redox function of APE1/Ref-1 is a novel strategy that might lead to a possible application of APX3330 for the treatment of IBD. | Versión del editor: | http://dx.doi.org/10.1093/ibd/izaa161 | URI: | http://hdl.handle.net/10261/261275 | DOI: | 10.1093/ibd/izaa161 | Identificadores: | doi: 10.1093/ibd/izaa161 issn: 1536-4844 |
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