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Título: | Oligonucleotide sensor based on magnetic capture and photoligation of upconverting nanoparticles in solid surfaces |
Autor: | Méndez-González, Diego; Silva-Ibáñez, Pedro P.; Valiente-Dies, Fernando; Calderon, Oscar G.; Méndez-González, Juan Luis CSIC; Laurenti, Marco; Egatz-Gómez, Ana; Díaz, Elena; Rubio-Retama, Jorge; Melle, Sonia | Palabras clave: | Upconverting nanoparticles Magnetic microparticles DNA detection |
Fecha de publicación: | 15-ago-2021 | Editor: | Elsevier | Citación: | Journal of Colloid and Interface Science 596: 64-74 (2021) | Resumen: | In this work, we present a luminescence platform that can be used as point of care system for determining the presence and concentration of specific oligonucleotide sequences. This sensor exhibited a limit of detection as low as 50 fM by means of: (i) the use of single-stranded DNA (ssDNA) functionalized magnetic microparticles that captured and concentrated ssDNA-upconverting nanoparticles (ssDNA-UCNPs) on a solid support, when the target sequence (miR-21-5p DNA-analogue) was in the sample, and (ii) a photoligation reaction that covalently linked the ssDNA-UCNPs and the ssDNA magnetic microparticles, allowing stringent washes. The presented sensor showed a similar limit of detection when the assays were conducted in samples containing total miRNA extracted from human serum, demonstrating its suitability for detecting small specific oligonucleotide sequences under real-like conditions. The strategy of combining UCNPs, magnetic microparticles, and a photoligation reaction provides new insight into low-cost, rapid, and ultra-sensitive detection of oligonucleotide sequences. | Descripción: | 11 pags., 7 figs., 1 tab. | Versión del editor: | http://dx.doi.org/10.1016/j.jcis.2021.02.093 | URI: | http://hdl.handle.net/10261/259177 | DOI: | 10.1016/j.jcis.2021.02.093 | Identificadores: | doi: 10.1016/j.jcis.2021.02.093 issn: 1095-7103 |
Aparece en las colecciones: | (CFMAC-IO) Artículos (ICMM) Artículos |
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