Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/258418
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Dynamics of aqueous peptide solutions in folded and disordered states examined by dynamic light scattering and dielectric spectroscopy |
Autor: | Melillo, Jorge H. CSIC ORCID; Gabriel, Jan Philipp; Pabst, Florian; Blochowicz, Thomas; Cerveny, Silvina CSIC ORCID | Fecha de publicación: | 2021 | Editor: | Royal Society of Chemistry (UK) | Citación: | Physical Chemistry Chemical Physics 23(28): 15020-15029 (2021) | Resumen: | Characterizing the segmental dynamics of proteins, and intrinsically disordered proteins in particular, is a challenge in biophysics. In this study, by combining data from broadband dielectric spectroscopy (BDS) and both depolarized (DDLS) and polarized (PDLS) dynamic light scattering, we were able to determine the dynamics of a small peptide [ε-poly(lysine)] in water solutions in two different conformations (pure β-sheet at pH = 10 and a more disordered conformation at pH = 7). We found that the segmental (α-) relaxation, as probed by DDLS, is faster in the disordered state than in the folded conformation. The water dynamics, as detected by BDS, is also faster in the disordered state. In addition, the combination of BDS and DDLS results allows us to confirm the molecular origin of water-related processes observed by BDS. Finally, we discuss the origin of two slow processes (A and B processes) detected by DDLS and PDLS in both conformations and usually observed in other types of water solutions. For fully homogeneous ε-PLL solutions at pH = 10, the A-DLS process is assigned to the diffusion of individual β-sheets. The combination of both techniques opens a route for understanding the dynamics of peptides and other biological solutions. | Versión del editor: | https://doi.org/10.1039/D1CP01893K | URI: | http://hdl.handle.net/10261/258418 | DOI: | 10.1039/D1CP01893K | E-ISSN: | 1463-9076 |
Aparece en las colecciones: | (CFM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
dynamistrep.pdf | 6,98 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
WEB OF SCIENCETM
Citations
3
checked on 27-feb-2024
Page view(s)
33
checked on 13-may-2024
Download(s)
52
checked on 13-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons