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http://hdl.handle.net/10261/251186
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Alonso, M. Carmen | - |
dc.contributor.author | Cano Cejas, Irene | - |
dc.contributor.author | Castro, Dolores | - |
dc.contributor.author | Pérez Prieto, Sara I. | - |
dc.contributor.author | Borrego, Juan J. | - |
dc.date.accessioned | 2021-09-29T07:30:12Z | - |
dc.date.available | 2021-09-29T07:30:12Z | - |
dc.date.issued | 2004-03-15 | - |
dc.identifier | doi: 10.1016/j.jviromet.2003.11.003 | - |
dc.identifier | issn: 0166-0934 | - |
dc.identifier.citation | Journal of Virological Methods 116(2): 133-138 (2004) | - |
dc.identifier.uri | http://hdl.handle.net/10261/251186 | - |
dc.description.abstract | An in situ hybridisation (ISH) technique has been developed to detect sole aquabirnavirus in infected fish cell lines bluegill fibroblast (BF-2), EPC, and chinook salmon embryo cells (CHSE-214). A 613bp cDNA probe for viral RNA coding for a fragment of VP2 protein was generated by reverse transcription polymerase chain reaction (RT-PCR) using infectious pancreatic necrosis virus (IPNV) specific DNA primers. Infected cells were strongly labelled, and no non-specific reaction was observed in non-infected cells used as negative controls. The specificity of the probe was examined by testing it against a range of IPNV serotypes such as Ab, Sp and VR-299. The ISH technique was compared with the immunofluorescence procedure to determine the sensitivity of detection of sole aquabirnavirus in BF-2 cells. The probe used in the ISH technique detected weak positivity at 8h post-inoculation (p.i.) in the cytoplasm of infected BF-2 cells inoculated with 103 TCID 50/ml, whilst the labelling appears at 24h p.i. when the immunofluorescence technique was applied. At all other time intervals the results were equivalent. | - |
dc.description.sponsorship | This study was supported by a grant of CICYT of the Spanish Government (MAR99-0609). | - |
dc.language | eng | - |
dc.publisher | Elsevier BV | - |
dc.rights | closedAccess | - |
dc.subject | Immunofluorescence | - |
dc.subject | In situ hybridisation | - |
dc.subject | Sole aquabirnavirus | - |
dc.subject | Tissue culture | - |
dc.title | Development of an in situ hybridisation procedure for the detection of sole aquabirnavirus in infected fish cell cultures | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.jviromet.2003.11.003 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.jviromet.2003.11.003 | - |
dc.date.updated | 2021-09-29T07:30:12Z | - |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
item.fulltext | No Fulltext | - |
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