Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/226495
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Genomic nucleotide sequence of a foot-and-mouth disease virus clone and its persistent derivatives: Implications for the evolution of viral quasispecies during a persistent infection |
Autor: | Toja, Miguel; Escarmís, Cristina ; Domingo, Esteban CSIC ORCID | Palabras clave: | Coevolution Mutation frequency Persisten infection Viral phenotype |
Fecha de publicación: | nov-1999 | Editor: | Elsevier | Citación: | Virus Research 64(2): 161-171 (1999) | Resumen: | The consensus nucleotide sequence of the entire genome of foot-and-mouth disease virus (FMDV) (biological clone C-S8c1) has been completed, and compared with that of two persistent derivatives R99 and R146, rescued after 99 and 146 passages of the carrier BHK-21 cells. Consensus sequences were determined directly from supernatants of persistently infected cells, without intervening cytolytic amplification of the viruses. These genomic sequences have also been compared with that of FMDV R100, a virus that was also rescued from persistently infected cells, but that was subjected to cytolytic amplification prior to sequencing. Mutation frequencies for R99 and R146 relative to C-S8c1 were in the range of 2.8x10 to 7.7x10 substitutions per nucleotide for the 5'-UTR and the L-, P1-, P2- and P3-coding regions. No mutations were fixed in the polymerase (3D)-coding region. Striking contrasts were noted regarding the distribution of mutation types along the persistent genomes, notably the complete absence of transversion mutations within the 5'-UTR, compared with 53% transversions in the L- and P1-coding regions. The sequencing results presented here, combined with previous sequences of FMDV C-S8c1 genomes at the onset of persistence, provide evidence of sequence fluctuations with a non-linear accumulation of mutations during prolonged persistence, a hallmark of quasispecies dynamics. Copyright (C) 1999 Elsevier Science B.V. | Versión del editor: | http://doi.org/10.1016/S0168-1702(99)00089-1 | URI: | http://hdl.handle.net/10261/226495 | DOI: | 10.1016/S0168-1702(99)00089-1 | Identificadores: | doi: 10.1016/S0168-1702(99)00089-1 issn: 0168-1702 |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
77
checked on 03-may-2024
WEB OF SCIENCETM
Citations
71
checked on 28-feb-2024
Page view(s)
85
checked on 16-may-2024
Download(s)
8
checked on 16-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.