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http://hdl.handle.net/10261/222491
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Perdomo, Germán | es_ES |
dc.contributor.author | Commerford, S. Renee | es_ES |
dc.contributor.author | Richard, Ann-Marie T. | es_ES |
dc.contributor.author | Adams, Sean H. | es_ES |
dc.contributor.author | Corkey, Barbara E. | es_ES |
dc.contributor.author | O'Doherty, Robert M. | es_ES |
dc.contributor.author | Brown, Nicholas F. | es_ES |
dc.date.accessioned | 2020-11-06T14:38:16Z | - |
dc.date.available | 2020-11-06T14:38:16Z | - |
dc.date.issued | 2004-06-25 | - |
dc.identifier.citation | The Journal of Biological Chemistry 279: 27177-27186 (2004) | es_ES |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10261/222491 | - |
dc.description.abstract | Skeletal muscle insulin resistance may be aggravated by intramyocellular accumulation of fatty acid-derived metabolites that inhibit insulin signaling. We tested the hypothesis that enhanced fatty acid oxidation in myocytes should protect against fatty acid-induced insulin resistance by limiting lipid accumulation. L6 myotubes were transduced with adenoviruses encoding carnitine palmitoyltransferase I (CPT I) isoforms or β-galactosidase (control). Two to 3-fold overexpression of L-CPT I, the endogenous isoform in L6 cells, proportionally increased oxidation of the long-chain fatty acids palmitate and oleate and increased insulin stimulation of [14C]glucose incorporation into glycogen by 60% while enhancing insulin-stimulated phosphorylation of p38MAPK. Incubation of control cells with 0.2 mM palmitate for 18 h caused accumulation of triacylglycerol, diacylglycerol, and ceramide (but not long-chain acyl-CoA) and decreased insulin-stimulated [14C]glucose incorporation into glycogen (60%), [3H]deoxyglucose uptake (60%), and protein kinase B phosphorylation (20%). In the context of L-CPT I overexpression, palmitate preincubation produced a relative decrease in insulin-stimulated incorporation of [14C]glucose into glycogen (60%) and [3H]deoxyglucose uptake (40%) but did not inhibit phosphorylation of protein kinase B. Due to the enhancement of insulin-stimulated glucose metabolism induced by L-CPT I overexpression itself, net insulin-stimulated incorporation of [14C]glucose into glycogen and [3H]deoxyglucose uptake in L-CPT I-transduced, palmitate-treated cells were significantly greater than in palmitate-treated control cells (71 and 75% greater, respectively). However, L-CPT I overexpression failed to decrease intracellular triacylglycerol, diacylglycerol, ceramide, or long-chain acyl-CoA. We propose that accelerated β-oxidation in muscle cells exerts an insulin-sensitizing effect independently of changes in intracellular lipid content. | es_ES |
dc.language.iso | eng | es_ES |
dc.rights | closedAccess | es_ES |
dc.title | Increased β-Oxidation in Muscle Cells Enhances Insulin-stimulated Glucose Metabolism and Protects against Fatty Acid-induced Insulin Resistance Despite Intramyocellular Lipid Accumulation | es_ES |
dc.type | artículo | es_ES |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1074/jbc.M403566200 | es_ES |
dc.identifier.e-issn | 1083-351X | - |
dc.relation.csic | No | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
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