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http://hdl.handle.net/10261/221805
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Madariaga-Marcos, Julene | es_ES |
dc.contributor.author | Corti, Roberta | es_ES |
dc.contributor.author | Hormeño, Silvia | es_ES |
dc.contributor.author | Moreno-Herrero, Fernando | es_ES |
dc.date.accessioned | 2020-10-26T12:10:17Z | - |
dc.date.available | 2020-10-26T12:10:17Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.citation | Scientific Reports 10 (1): 18069 (2020) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/221805 | - |
dc.description.abstract | Single-molecule experiments usually take place in flow cells. This experimental approach is essential for experiments requiring a liquid environment, but is also useful to allow the exchange of reagents before or during measurements. This is crucial in experiments that need to be triggered by ligands or require a sequential addition of proteins. Home-fabricated flow cells using two glass coverslips and a gasket made of paraffin wax are a widespread approach. The volume of the flow cell can be controlled by modifying the dimensions of the channel while the reagents are introduced using a syringe pump. In this system, high flow rates disturb the biological system, whereas lower flow rates lead to the generation of a reagent gradient in the flow cell. For very precise measurements it is thus desirable to have a very fast exchange of reagents with minimal diffusion. We propose the implementation of multistream laminar microfluidic cells with two inlets and one outlet, which achieve a minimum fluid switching time of 0.25 s. We additionally define a phenomenological expression to predict the boundary switching time for a particular flow cell cross section. Finally, we study the potential applicability of the platform to study kinetics at the single molecule level. | es_ES |
dc.description.sponsorship | European Research Council (ERC) under the European Union Horizon 2020 research and innovation (grant agreement No 681299). The work in the Moreno-Herrero laboratory was also supported by a Spanish Ministry of Economy and Competitiveness Grant, BFU2017-83794-P (AEI/FEDER, UE) to F.M.-H., and Comunidad de Madrid Grants, Tec4Bio – S2018/NMT-4443 and NanoBioCancer – Y2018/BIO-4747 to F.M.-H. J.M.-M. acknowledges support for pre-doctoral fellowship from the Basque Country Government Department of Education, Language Policy and Culture (ref. PRE_2013_11_1174). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Characterizing microfluidic approaches for a fast and efficient reagent exchange in single-molecule studies | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1038/s41598-020-74523-w | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1038/s41598-020-74523-w | es_ES |
dc.identifier.e-issn | 2045-2322 | - |
dc.contributor.funder | European Research Council | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | Comunidad de Madrid | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000781 | es_ES |
dc.contributor.orcid | Madariaga-Marcos, Julene [0000-0003-1458-3369] | es_ES |
dc.contributor.orcid | Corti, Roberta [0000-0002-4737-7900] | es_ES |
dc.contributor.orcid | Hormeño, Silvia [0000-0001-6549-357X] | es_ES |
dc.contributor.orcid | Morneo-Herrero, Fernando [0000-0003-4083-1709] | es_ES |
dc.identifier.pmid | 33093484 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
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Scientific Reports - 2020.pdf | Artículo principal | 3,56 MB | Adobe PDF | Visualizar/Abrir |
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