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Título: | Rescue of SARS-CoV-2 from a Single Bacterial Artificial Chromosome |
Autor: | Ye, Chengjin; Chiem, Kevin; Park, Jun-Gyu; Oladunni, Fatai; Platt, Roy Neal; Anderson, Tim; Almazán, Fernando CSIC ORCID ; Torre, Juan Carlos de la; Martínez-Sobrido, Luis | Palabras clave: | BAC COVID-19 SARS-CoV-2 Coronavirus Hamsters Recombinant virus Reverse genetics |
Fecha de publicación: | 25-sep-2020 | Editor: | American Society for Microbiology | Citación: | mBio 11(5): e02168-20 (2020) | Resumen: | Infectious coronavirus (CoV) disease 2019 (COVID-19) emerged in the city of Wuhan (China) in December 2019, causing a pandemic that has dramatically impacted public health and socioeconomic activities worldwide. A previously unknown coronavirus, severe acute respiratory syndrome CoV-2 (SARS-CoV-2), has been identified as the causative agent of COVID-19. To date, there are no U.S. Food and Drug Administration (FDA)-approved vaccines or therapeutics available for the prevention or treatment of SARS-CoV-2 infection and/or associated COVID-19 disease, which has triggered a large influx of scientific efforts to develop countermeasures to control SARS-CoV-2 spread. To contribute to these efforts, we have developed an infectious cDNA clone of the SARS-CoV-2 USA-WA1/2020 strain based on the use of a bacterial artificial chromosome (BAC). Recombinant SARS-CoV-2 (rSARS-CoV-2) was readily rescued by transfection of the BAC into Vero E6 cells. Importantly, BAC-derived rSARS-CoV-2 exhibited growth properties and plaque sizes in cultured cells comparable to those of the natural SARS-CoV-2 isolate. Likewise, rSARS-CoV-2 showed levels of replication similar to those of the natural isolate in nasal turbinates and lungs of infected golden Syrian hamsters. This is, to our knowledge, the first BAC-based reverse genetics system for the generation of infectious rSARS-CoV-2 that displays features in vivo similar to those of a natural viral isolate. This SARS-CoV-2 BAC-based reverse genetics will facilitate studies addressing several important questions in the biology of SARS-CoV-2, as well as the identification of antivirals and development of vaccines for the treatment of SARS-CoV-2 infection and associated COVID-19 disease. | Versión del editor: | https://doi.org/10.1128/mBio.02168-20 | URI: | http://hdl.handle.net/10261/220489 | DOI: | 10.1128/mBio.02168-20 | E-ISSN: | 2150-7511 |
Aparece en las colecciones: | (PTI Salud Global) Colección Especial COVID-19 (CNB) Artículos |
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Fichero | Descripción | Tamaño | Formato | |
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Rescue of SARSCoV2 from a Single Bacterial Artificial.pdf | 1,92 MB | Adobe PDF | Visualizar/Abrir |
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