Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/207699
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Zinc Differentially Modulates the Assembly of Soluble and Polymerized Vimentin |
Autor: | Mónico, Andreia; Zorrilla, Silvia CSIC ORCID ; Rivas, Germán CSIC ORCID CVN ; Pérez-Sala, Dolores CSIC ORCID | Palabras clave: | Vimentin Zinc Cysteine Redox sensing Intermediate filaments Cysteine mutant Filament bundling Filament width Divalent cations Magnesium |
Fecha de publicación: | 2020 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | International Journal of Molecular Sciences 21(7): 2426 (2020) | Resumen: | The intermediate filament protein vimentin constitutes a critical sensor for electrophilic and oxidative stress. We previously showed that vimentin interacts with zinc, which affects its assembly and redox sensing. Here, we used vimentin wt and C328S, an oxidation-resistant mutant showing improved NaCl-induced polymerization, to assess the impact of zinc on soluble and polymerized vimentin by light scattering and electron microscopy. Zinc acts as a switch, reversibly inducing the formation of vimentin oligomeric species. High zinc concentrations elicit optically-detectable vimentin structures with a characteristic morphology depending on the support. These effects also occur in vimentin C328S, but are not mimicked by magnesium. Treatment of vimentin with micromolar ZnCl2 induces fibril-like particles that do not assemble into filaments, but form aggregates upon subsequent addition of NaCl. In contrast, when added to NaCl-polymerized vimentin, zinc increases the diameter or induces lateral association of vimentin wt filaments. Remarkably, these effects are absent or attenuated in vimentin C328S filaments. Therefore, the zinc-vimentin interaction depends on the chemical environment and on the assembly state of the protein, leading to atypical polymerization of soluble vimentin, likely through electrostatic interactions, or to broadening and lateral association of preformed filaments through mechanisms requiring the cysteine residue. Thus, the impact of zinc on vimentin assembly and redox regulation is envisaged. | Descripción: | © 2020 by the authors. | Versión del editor: | https://doi.org/10.3390/ijms21072426 | URI: | http://hdl.handle.net/10261/207699 | DOI: | 10.3390/ijms21072426 | E-ISSN: | 1422-0067 |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Zinc_Monico_Art2020.pdf | 8,49 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
12
checked on 16-abr-2024
SCOPUSTM
Citations
14
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
13
checked on 23-feb-2024
Page view(s)
118
checked on 27-abr-2024
Download(s)
131
checked on 27-abr-2024