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Title

Uptake and biological effects of the insecticide Fipronil on seabass

AuthorsDallarés, Sara ; Dourado, Priscila; Montemurro, Nicola; Pérez Solsona, Sandra ; Berdié, L.; Solé, Montserrat
KeywordsFipronil
Seabass
Oxidative stress
CYP metabolism
Enzymatic marker
Issue Date10-Jun-2019
Citation17th International Conference on Chemistry and the Environment : Conference proceedings: 852-854 (2019)
AbstractThe phenylpyrazole Fipronil is one of the most widely used insecticides in crops worldwide. Its use was banned by the European Union in April/2013 (Directive ECC Nº 781/2013) due to its harmful effects to non-target species (Gripp et al., 2017; Schlenk et al., 2001; Stefani Margarido et al., 2013). Despite this, Spain, the largest Fipronil consumer in Europe in sunflower crops, is still reluctant to adhere to the European directive alluding to few on-site studies that proved their toxicity. The aim of the present study was to assess a suite of biomarker responses on seabass (Dicentrarchus labrax) exposed through diet to Fipronil under two environmentally-realistic temperature regimes. Juveniles of D. labrax were acclimated (14 days) to two temperature regimes of 13 and 16 ºC. Then, fish were fed spiked food with 10 mg/Kg of Regent®800WG (80% Fipronil) during two weeks, after which a depuration period of one week (feeding with unspiked food) was allowed. Eight fish were sampled at each temperature before Fipronil exposure (t0), after 7 and 14 days exposure (t7 and t14) and after a 7-day depuration period (t21), and dissected according to a standardized protocol. Feeding rate was calculated for each condition (temperature × time) and morphometric measurements were recorded for each fish. Concentration of Fipronil and its main metabolite Fipronil sulfone was determined in bile by LC-HRMS and in muscle (only Fipronil) by GC-MS. Metabolic and oxidative-stress related markers were assessed in different tissues: lactate dehydrogenase (LDH), six Cytochrome P450-related (CYP) enzymes, NAD(P)H cyt c and NADH ferricyanide reductases, uridine diphosphate glucuronyltransferase (UDPGT) and glutathione S-transferase (GST) and the antioxidant enzymatic responses catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GPX) activities, as well as the marker indicative of oxidative stress damage: lipid peroxidation (LPO) levels. Differences between temperatures and time-groups (t0, t7, t14 and t21) were tested by ANOVA contrasts
Description17th International Conference on Chemistry and the Environment (ICCE 2019), 16-20 June 2019, Thessaloniki, Greece.-- 3 pages, 2 figures, 2 tables
URIhttp://hdl.handle.net/10261/206011
Appears in Collections:(IDAEA) Comunicaciones congresos
(ICM) Comunicaciones congresos
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