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http://hdl.handle.net/10261/192536
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dc.contributor.author | Nieto-Domínguez, Manuel José | - |
dc.contributor.author | Martínez-Fernández, José Alberto | - |
dc.contributor.author | Fernández de Toro, Beatriz | - |
dc.contributor.author | Méndez-Líter, Juan A. | - |
dc.contributor.author | Cañada, F. Javier | - |
dc.contributor.author | Prieto Orzanco, Alicia | - |
dc.contributor.author | Eugenio, Laura I. de | - |
dc.contributor.author | Martínez, María Jesús | - |
dc.date.accessioned | 2019-10-13T18:11:54Z | - |
dc.date.available | 2019-10-13T18:11:54Z | - |
dc.date.issued | 2019-10-10 | - |
dc.identifier.citation | Microbial Cell Factories 18(1): 174 (2019) | - |
dc.identifier.uri | http://hdl.handle.net/10261/192536 | - |
dc.description.abstract | [Background] Currently, industrial societies are seeking for green alternatives to conventional chemical synthesis. This demand has merged with the efforts to convert lignocellulosic biomass into value-added products. In this context, xylan, as one of main components of lignocellulose, has emerged as a raw material with high potential for advancing towards a sustainable economy. | - |
dc.description.abstract | [Results] In this study, the recombinant endoxylanase rXynM from the ascomycete Talaromyces amestolkiae has been heterologously expressed in Pichia pastoris and used as one of the catalysts of an enzyme cascade developed to synthesize the antiproliferative 2-(6-hydroxynaphthyl) β-d-xylopyranoside, by transglycosylation of 2,6-dihydroxynaphthalene. The approach combines the use of two fungal xylanolytic enzymes, rXynM and the β-xylosidase rBxTW1 from the same fungus, with the cost-effective substrate xylan. The reaction conditions for the cascade were optimized by a Central Composite Design. Maximal productions of 0.59 and 0.38 g/L were reached using beechwood xylan and birchwood xylan, respectively. For comparison, xylans from other sources were tested in the same reaction, suggesting that a specific optimization is required for each xylan variety. The results obtained using this enzyme cascade and xylan were similar or better to those previously reported for a single catalyst and xylobiose, an expensive sugar donor. | - |
dc.description.abstract | [Conclusions] Beechwood and birchwood xylan, two polysaccharides easily available from biomass, were used in a novel enzyme cascade to synthetize an antiproliferative agent. The approach represents a green alternative to the conventional chemical synthesis of 2-(6-hydroxynaphthyl) β-d-xylopyranoside using a cost-effective substrate. The work highlights the role of xylan as a raw material for producing value-added products and the potential of fungal xylanolytic enzymes in the biomass conversion. | - |
dc.description.sponsorship | We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). This work has been funded by projects BIO2015-68387-R, CTQ2015-64597-C2-2-P and RTI2018-093683-B-I00 from MINECO and S2018/EMT-4459 from Comunidad de Madrid. M. Nieto-Domínguez and Beatriz Fernández de Toro thank the MINECO for an FPU and an FPI fellowship, respectively. | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation | RTI2018-093683-B-I00/AEI/10.13039/501100011033 | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-68387-R | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-64597-C2-2-P | - |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093683-B-I00 | - |
dc.relation | S2018/EMT-4459/RETOPROSOST-2CM | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.subject | Endoxylanase | - |
dc.subject | β-Xylosidase | - |
dc.subject | Response surface methodology | - |
dc.subject | Transxylosylation | - |
dc.subject | Antiproliferative | - |
dc.title | Exploiting xylan as sugar donor for the synthesis of an antiproliferative xyloside using an enzyme cascade | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1186/s12934-019-1223-9 | - |
dc.description.peerreviewed | Peer reviewed | - |
dc.relation.publisherversion | https://doi.org/10.1186/s12934-019-1223-9 | - |
dc.identifier.e-issn | 1475-2859 | - |
dc.date.updated | 2019-10-13T18:11:55Z | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | Agencia Estatal de Investigación (España) | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011033 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.contributor.orcid | Martínez, María Jesús [0000-0003-2166-1097] | - |
dc.identifier.pmid | 31601204 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
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xylan_sugar_donor_synthesis_antiproliferative_xyloside_enzyme_cascade.pdf | 2 MB | Adobe PDF | Visualizar/Abrir |
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