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http://hdl.handle.net/10261/191120
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dc.contributor.author | Borisova, Boryana | - |
dc.contributor.author | Sánchez, Alfredo | - |
dc.contributor.author | Jiménez-Falcao, Sandra | - |
dc.contributor.author | Martín, Miriam | - |
dc.contributor.author | Salazar, Pedro | - |
dc.contributor.author | Parrado, Concepción | - |
dc.contributor.author | Pingarrón, José Manuel | - |
dc.contributor.author | Villalonga, Reynaldo | - |
dc.date.accessioned | 2019-09-20T07:53:24Z | - |
dc.date.available | 2019-09-20T07:53:24Z | - |
dc.date.issued | 2016-09 | - |
dc.identifier.citation | Sensors and Actuators - B - Chemical Biochemical Sensors 232: 84-90 (2016) | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/10261/191120 | - |
dc.description.abstract | The assembly of a novel layer-by-layer biosensor architecture using hybrid nanomaterials is explored for the construction of an amperometric enzyme biosensors. The nanostructured sensing interface was prepared with poly(dopamine)-modified magnetic nanoparticles which were covalently coated with four-generation ethylenediamine core polyamidoamine G-4 dendrimers and further decorated with platinum nanoparticles. This nanohybrid was fully characterized and further layered on glassy carbon electrodes coated with a graphene oxide-carboxymethylcellulose hybrid nanomaterial through electrostatic interactions. The nanostructured surface was then employed as scaffold for the covalent immobilization of the enzyme xanthine oxidase through a glutaraldehyde-mediated cross-linking. The enzyme electrode allowed the amperometric detection of xanthine in the 50 nM-12 μM range, with a high sensitivity of 140 mA/M cm and low detection limit of 13 nM. The biosensor exhibited high reproducibility and repeatability, and was successfully tested for the quantification of xanthine in fish samples. | - |
dc.description.sponsorship | Financial support from the Spanish Ministry of Economy and CompetitivenessCTQ2014-58989-P, CTQ2011-24355, CTQ2015-71936-REDT, CTQ2012-34238 and Comunidad de MadridS2013/MIT-3029, Programme NANOAVANSENS is gratefully acknowledged. | - |
dc.publisher | Elsevier | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-58989-P | - |
dc.relation | S2013/MIT-3029/NANOAVANSENS | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-71936-REDT | - |
dc.rights | closedAccess | - |
dc.subject | Graphene | - |
dc.subject | Hybrid nanomaterials | - |
dc.subject | Magnetic nanoparticles | - |
dc.subject | Biosensors | - |
dc.subject | Fish freshness | - |
dc.title | Reduced graphene oxide-carboxymethylcellulose layered with platinum nanoparticles/PAMAM dendrimer/magnetic nanoparticles hybrids. Application to the preparation of enzyme electrochemical biosensors | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.snb.2016.02.106 | - |
dc.relation.publisherversion | https://doi.org/10.1016/j.snb.2016.02.106 | - |
dc.date.updated | 2019-09-20T07:53:25Z | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
item.fulltext | No Fulltext | - |
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