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Designing continuous flow reaction of xylan hydrolysis for xylooligosaccharides production in packed-bed reactors using xylanase immobilized on methacrylic polymer-based supports

AuthorsRomero-Fernández, Maria; Moreno-Pérez, Sonia; Orrego, Alejandro H.; Martins de Oliveira, Sandro; Santamaría, Ramón I. ; Díaz, Margarita ; Guisán, José Manuel ; Rocha-Martín, Javier
KeywordsXylan hydrolysis
Enzyme immobilization
Bioprocess intensification
Flow reactor
Issue DateOct-2018
CitationBioresource Technology 266: 249-258 (2018)
AbstractThe present study focuses on the development and optimization of a packed-bed reactor (PBR) for continuous production of xylooligosaccharides (XOS) from xylan. For this purpose, three different methacrylic polymer-based supports (Relizyme R403/S, Purolite P8204F and Purolite P8215F) activated with glyoxyl groups were morphologically characterized and screened for the multipoint covalent immobilization of a xylanase. Based on its physical and mechanical properties, maximum protein loading and thermal stability, Relizyme R403/S was selected to set up a PRB for continuous production of XOS from corncob xylan. The specific productivity for XOS at 10 mL/min flow rate was 3277 gXOS genzyme−1 h−1 with a PBR. This PBR conserved >90% of its initial activity after 120 h of continuous operation.
Publisher version (URL)https://doi.org/10.1016/j.biortech.2018.06.070
Appears in Collections:(IBFG) Artículos
(ICP) Artículos
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