English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/163802
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Galactooligosaccharide Production from Pantoea anthophila Strains Isolated from “Tejuino”, a Mexican Traditional Fermented Beverage

AutorYáñez-Ñeco, Claudia; Rodríguez-Colinas, Bárbara ; Amaya-Delgado, Lorena; Ballesteros Olmo, Antonio ; Gschaedler, Anne; Plou Gasca, Francisco José ; Arrizón, Javier
Palabras clavePantoea anthophila
Permeabilized cells
β-galactosidase
Galactooligosaccharides
Transglycosylation
Prebiotics
Fecha de publicación22-ago-2017
EditorMultidisciplinary Digital Publishing Institute
CitaciónCatalysts 7: 242 (2017)
ResumenTwo Pantoea anthophila bacterial strains were isolated from “tejuino”, a traditional Mexican beverage, and studied as β-galactosidase producers for galactooligosaccharides synthesis. Using 400 g/L of lactose, 50 °C, and 15 U/mL of β-galactosidase activity with ethanol-permeabilized cells, the maximum galactooligosaccharides (GOS) yield determined by High performance anion exchange chromatography with pulse amperometric detection (HPAEC-PAD) was 136 g/L (34% w/w of total sugars) at 96% of lactose conversion for Bac 55.2 and 145 g/L (36% w/w of total sugars) at 94% of lactose conversion for Bac 69.1. The main synthesized products were the disaccharides allolactose [Gal-β(1 → 6)-Glc] and 6-galactobiose [Gal-β(1 → 6)-Gal], as well as the trisaccharides 3′-galactosyl-lactose [Gal-β(1 → 3)-Gal-β(1 → 4)-Glc], 6-galactotriose [Gal-β(1 → 6)-Gal-β(1 → 6)-Gal], 3′-galactosyl-allolactose [Gal-β(1 → 3)-Gal-β(1 → 6)-Glc], and 6′-galactosyl-lactose [Gal-β(1 → 6)-Gal-β(1 → 4)-Glc]. The β-galactosidases present in both strains showed a high transgalactosylation activity and formed principally β(1 → 3) and β(1 → 6) linkages. Considering the stability and bifidogenic properties of GOS containing such types of bonds, P. anthophila strains Bac 55.2 and Bac 69.1 possess a high potential as novel biocatalysts for prebiotic industrial production.
Versión del editorhttp://dx.doi.org/10.3390/catal7080242
URIhttp://hdl.handle.net/10261/163802
DOI10.3390/catal7080242
E-ISSN2073-4344
Aparece en las colecciones: (ICP) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
fermented_beverage_Yanez.pdf556,49 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.