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Título: | Probing the Rare Biosphere of the North-West Mediterranean Sea: An Experiment with High Sequencing Effort |
Autor: | Crespo, B. G. CSIC; Wallhead, Philip J.; Logares, Ramiro CSIC ORCID ; Pedrós-Alió, Carlos CSIC ORCID | Fecha de publicación: | jul-2016 | Editor: | Public Library of Science | Citación: | PLoS ONE 11(7): e0159195 (2016) | Resumen: | High-throughput sequencing (HTS) techniques have suggested the existence of a wealth of species with very low relative abundance: the rare biosphere. We attempted to exhaustively map this rare biosphere in two water samples by performing an exceptionally deep pyrosequencing analysis (~500,000 final reads per sample). Species data were derived by a 97% identity criterion and various parametric distributions were fitted to the observed counts. Using the best-fitting Sichel distribution we estimate a total species richness of 1,568–1,669 (95% Credible Interval) and 5,027–5,196 for surface and deep water samples respectively, implying that 84–89% of the total richness in those two samples was sequenced, and we predict that a quadrupling of the present sequencing effort would suffice to observe 90% of the total richness in both samples. Comparing the HTS results with a culturing approach we found that most of the cultured taxa were not obtained by HTS, despite the high sequencing effort. Culturing therefore remains a useful tool for uncovering marine bacterial diversity, in addition to its other uses for studying the ecology of marine bacteria | Descripción: | 16 pages, 3 figures, 3 tables, supporting information https://dx.doi.org/10.1371/journal.pone.0159195.s001 | Versión del editor: | https://dx.doi.org/10.1371/journal.pone.0159195 | URI: | http://hdl.handle.net/10261/135306 | DOI: | 10.1371/journal.pone.0159195 | Identificadores: | doi: 10.1371/journal.pone.0159195 issn: 1932-6203 e-issn: 1932-6203 |
Aparece en las colecciones: | (CNB) Artículos (ICM) Artículos |
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