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Title

Short-term exposure of nontumorigenic human bronchial epithelial cells to carcinogenic chromium(VI) compromises their respiratory capacity and alters their bioenergetic signature

AuthorsCerveia, Joana F:; Sánchez-Aragó, María ; Urbano, Ana M.; Cuezva, José M.
Issue Date23-Jun-2014
PublisherElsevier
CitationFEBS Open Bio 4: 594- 601 (2014)
AbstractPrevious studies on the impact of hexavalent chromium [Cr(VI)] on mammalian cell energetics revealed alterations suggestive of a shift to a more fermentative metabolism. Aiming at a more defined understanding of the metabolic effects of Cr(VI) and of their molecular basis, we assessed the impact of a mild Cr(VI) exposure on critical bioenergetic parameters (lactate production, oxygen consumption and intracellular ATP levels). Cells derived from normal human bronchial epithelium (BEAS-2B cell line), the main in vivo target of Cr(VI) carcinogenicity, were subjected for 48h to 1¿M Cr(VI). We could confirm a shift to a more fermentative metabolism, resulting from the simultaneous inhibition of respiration and stimulation of glycolysis. This shift was accompanied by a decrease in the protein levels of the catalytic subunit (subunit ß) of the mitochondrial H+-ATP synthase (ß-F1-ATPase) and a concomitant marked increase in those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The corresponding alteration in the ß-F1-ATPase/GAPDH protein ratio (viewed as a bioenergetic signature) upon Cr(VI) exposure was in agreement with the observed attenuation of cellular respiration and enhancement of glycolytic flux. Altogether, these results constitute a novel finding in terms of the molecular mechanisms of Cr(VI) effects. © 2014 The Authors.
URIhttp://hdl.handle.net/10261/125472
DOI10.1016/j.fob.2014.06.006
Identifiersdoi: 10.1016/j.fob.2014.06.006
issn: 2211-5463
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