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dc.contributor.authorGarcía-Sacristán, Anaes_ES
dc.contributor.authorMoreno Molina, Migueles_ES
dc.contributor.authorAriza-Mateos, Ascensiónes_ES
dc.contributor.authorLópez-Camacho, Elenaes_ES
dc.contributor.authorJáudenes, Rosa M.es_ES
dc.contributor.authorVázquez, Luises_ES
dc.contributor.authorGómez, Jordies_ES
dc.contributor.authorMartín-Gago, José A.es_ES
dc.contributor.authorBriones, Carloses_ES
dc.identifier.citationNucleic Acids Researches_ES
dc.descriptionSupplementary data.es_ES
dc.description.abstractThe 5′ untranslated region of hepatitis C virus (HCV) genomic RNA contains an internal ribosome entry site (IRES) element, composed of domains II–IV, which is required for cap-independent translation initiation. Little information on the 3D structure of the whole functional HCV IRES is still available. Here, we use atomic force microscopy to visualize the HCV IRES conformation in its natural sequence context, which includes the upstream domain I and the essential, downstream domains V and VI. The 574 nt-long molecule analyzed underwent an unexpected, Mg2+-induced switch between two alternative conformations: from ‘open’, elongated morphologies at 0–2 mM Mg2+ concentration to a ‘closed’, comma-shaped conformation at 4–6 mM Mg2+. This sharp transition, confirmed by gel-shift analysis and partial RNase T1 cleavage, was hindered by the microRNA miR-122. The comma-shaped IRES-574 molecules visualized at 4–6 mM Mg2+ in the absence of miR-122 showed two arms. Our data support that the first arm would contain domain III, while the second one would be composed of domains (I–II)+(V–VI) thanks to a long-range RNA interaction between the I-II spacer and the basal region of domain VI. This reinforces the previously described structural continuity between the HCV IRES and its flanking domains I, V and VI.es_ES
dc.description.sponsorshipSpanish Ministerio de Ciencia e Innovación and Ministerio de Economía y Competitividad [BIO2010–20696 and BIO2013–47228-R to C.B.; MAT2011–26534 to J.A.M.- G.; BFU2010–11612-E to J.G.; FIS2012–38866-C05–05 to L.V.]; Spanish National Research Council [CSIC- 200920I040 to C.B.]; Comunidad de Madrid [NANOAVANSENS S2013/MIT-3029 to L.V.]; FEDER funds from the European Union. CIBERehd is funded by the Instituto de Salud Carlos III. JAE-Doc postdoctoral fellowship from the CSIC Program Junta de Ampliación de Estudios, co-funded by the European Science Foundation [to M.M.]. Funding for open access charge: Spanish NationalResearch Council (CSIC).es_ES
dc.publisherOxford University Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleA magnesium-induced RNA conformational switch at the internal ribosome entry site of hepatitis C virus genome visualized by atomic force microscopyes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.rights.licenseThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly citedes_ES
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