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Low-intensity pump-probe spectroscopy on the B800 to B850 transfer in the light harvesting 2 complex of Rhodobacter sphaeroides

AutorMonshouwer, R.; Ortiz de Zarate Avila, Iñaki; Mourik, F. van; Grondelle, R. van
Fecha de publicación1995
EditorElsevier
CitaciónChemical Physics Letters 246: 341-346 (1995)
ResumenThe isolated LH2 (B800-850) complex of Rb. sphaeroides has been studied at 77 K using low-intensity one-colour pump-probe spectroscopy. Delta absorbance transients were measured at several wavelengths within the B800 band. In the red part of the band, the excited B800 population decays mono-exponentially with a lifetime of about 1.2 ± 0.1 ps. More to the blue much faster rates are found which are ascribed to downhill energy-transfer among the B800 pigments. To our surprise, increasing the average intensity from 4 W/cm2 to higher excitation densities significantly slows the decay of B800*. Since this effect is permanent we conclude that it is due to some form of photodamage. We propose that this observation explains the slower decay reported by others. © 1995.
URIhttp://hdl.handle.net/10261/121158
DOInull
Identificadoresdoi: null
issn: 0009-2614
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