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Título: | Increased superoxide anion production by interleukin-1β impairs nitric oxide-mediated relaxation in resistance arteries |
Autor: | Jiménez-Altayó, Francesc; Briones, Ana M.; Giraldo, Jesús; Planas, Anna M. CSIC ORCID; Salaices, Mercedes; Vila, Elisabet | Fecha de publicación: | ene-2006 | Editor: | American Society for Pharmacology and Experimental Therapeutics | Citación: | Journal of Pharmacology and Experimental Therapeutics 316(1): 42-52 (2006) | Resumen: | The present study was designed to analyze the effect of long-term incubation with interleukin-1β (IL-1β) on endothelium-dependent relaxation in rat mesenteric resistance arteries. Vessels were incubated in culture medium with or without IL-1β (10 ng/ml, 14 h). Changes in lumen diameter were recorded in a pressure myograph. Protein expression, nitrite, and superoxide anion (O2 .-) production were evaluated by either Western blot or immunofluorescence, Griess reaction, and ethidium fluorescence, respectively. IL-1β impaired acetylcholine (ACh) and sodium nitroprusside (SNP) vasodilation and increased nitrite and O2 .- production and inducible nitric-oxide synthase (iNOS), xanthine oxidase, and p22phox expression. However, neither endothelial nitric-oxide synthase (NOS) nor soluble guanylate cyclase protein expression were affected by IL-1β treatment. Polyethylene glycol superoxide dismutase partially reversed the impairment of ACh relaxation and abolished the O 2 .- production observed in IL-1β-treated arteries. The impairment of ACh relaxation induced by IL-1β was also partially reversed by the xanthine oxidase inhibitor allopurinol (1 mM) but not by either the NADPH oxidase inhibitor apocynin (0.3 mM) or the inducible NOS inhibitor N-3-aminomethylbenzylacetamidine (1 μM). However, all these inhibitors improved the impaired SNP response. The results of the present study demonstrate that long-term incubation with IL-1β induces an impairment of the nitric oxide-mediated relaxation in mesenteric resistance arteries through the production of O2 .-, mainly from xanthine oxidase. Copyright © 2006 by The American Society for Pharmacology and Experimental Therapeutics. | Descripción: | Part of this work was presented at the 8th International Symposium on Resistance Arteries, Angers, France, June 20–23, 2004 | Versión del editor: | http://dx.doi.org/10.1124/jpet.105.088435 | URI: | http://hdl.handle.net/10261/116742 | DOI: | 10.1124/jpet.105.088435 | Identificadores: | doi: 10.1124/jpet.105.088435 issn: 0022-3565 |
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