English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/11068
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Identification of Pratylenchus thornei, the cereal and legume root-lesion nematode, based on SCAR-PCR and satellite DNA

AuthorsCarrasco-Ballesteros, Susana; Castillo, Pablo ; Adams, Byron J.; Pérez-Artés, Encarnación
KeywordsMolecular diagnosis
Plant-parasitic nematodes
Sequence characterized amplified region
satDNA
Issue Date2-Jun-2007
PublisherSpringer
CitationEuropean Journal of Plant Patholology (2007) 118:115–125
AbstractTwo different molecular tools for the diagnosis of the cereal and legume root-lesion nematode Pratylenchus thornei were developed. A randomly amplified DNA (RAPD) fragment specific to P. thornei was identified. After sequencing the fragment, longer primers were designed that complement the terminal sequences of the RAPD fragment, and this pair of specific primers was used to amplify the sequence-characterized amplified region (SCAR). Using the developed pair of SCAR primers, the SCAR fragment specific to P. thornei was easily amplified with DNA extracts obtained from different life stages of the nematode. The described SCARPCR- based assay has the potential to be optimized for routine practical diagnostic tests. In addition, the use of a species-specific satellite DNA sequence to distinguish P. thornei from other Pratylenchus spp. is discussed.
Publisher version (URL)http://dx.doi.org/10.1007/s10658-007-9110-3
URIhttp://hdl.handle.net/10261/11068
DOI10.1007/s10658-007-9110-3
ISSN0929-1873
E-ISSN1573-8469
Appears in Collections:(IAS) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.