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Título: | Root nodule enzymes of ammonia metabolism from medicago sativa L. as influenced by nitrate levels |
Autor: | Becana Ausejo, Manuel CSIC ORCID ; Aparicio-Tejo, Pedro María CSIC; Sánchez-Díaz, Manuel CSIC ORCID | Palabras clave: | Medicago sativa enzymes o fammonia assimilation nitrate levels nitrogenase activity nodule senescence |
Fecha de publicación: | 1984 | Editor: | Elsevier | Citación: | Journal of Plant Physiology 116 (4): 285–292 (1984) | Resumen: | Plants of alfalfa (Medicago sativa L.) were subjected to six levels of nitrate ranging from 0 to 200 mM. Then, nodule content of soluble protein and activities of the nodule enzymes, nitrogenase (C2H2 reduction activity), glutamine synthetase (EC 6.3.1.2), glutamate synthase (EC 1.4.1.14), and NADH/NAD+-glutamate dehydrogenases (EC 1.4.1.2) were determined. Decline of glutamate synthase activity closely paralleled that of nitrogenase. In contrast, glutamine synthetase activity reached a minimum at 20mM nitrate but began to recover at higher nitrate concentrations. Patterns of NADH-(aminating) and NAD+-(deaminating) glutamate dehydrogenase activities were opposed with a great increase (2- to 5-fold) in the aminating direction at highest levels of nitrate. Results indicate a main role of glutamine synthetase/glutamate synthase pathway in the assimilation of fixed N. In addition, increase of the activities of glutamine synthetase and NADH-glutamate dehydrogenase concomitant with an almost fully suppressed nitrogenase and decreased NAD+-glutamate dehydrogenase activities at above 20mM nitrate reflect: (a) rapid induced senescence of nodules and (b) the production of ammonia by alternative means as nitrate reduction or oxidative deamination of amino acids. | Descripción: | 8 Pags. | Versión del editor: | http://dx.doi.org/10.1016/S0176-1617(84)80107-8 | URI: | http://hdl.handle.net/10261/109205 | DOI: | 10.1016/S0176-1617(84)80107-8 | ISSN: | 0176-1617 |
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