Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/10491
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Navarro, José A. | - |
dc.contributor.author | Lowe, Christian E. | - |
dc.contributor.author | Amons, Reinout | - |
dc.contributor.author | Kohzuma, Takamitsu | - |
dc.contributor.author | Canters, Gerard W. | - |
dc.contributor.author | Rosa, Miguel A. de la | - |
dc.contributor.author | Ubbink, Marcellus | - |
dc.contributor.author | Hervás, Manuel | - |
dc.date.accessioned | 2009-02-10T09:49:36Z | - |
dc.date.available | 2009-02-10T09:49:36Z | - |
dc.date.issued | 2004-07-30 | - |
dc.identifier.citation | European Journal of Biochemistry 271(16): 3449-3456 (2004) | en_US |
dc.identifier.issn | 0014-2956 | - |
dc.identifier.uri | http://hdl.handle.net/10261/10491 | - |
dc.description | 8 pages, 7 figures.-- PMID: 15291822 [PubMed]. | en_US |
dc.description.abstract | Plastocyanin (Pc) is a soluble copper protein that transfers electrons from cytochrome b6f to photosystem I (PSI), two protein complexes that are localized in the thylakoid membranes in chloroplasts. The surface electrostatic potential distribution of Pc plays a key role in complex formation with the membrane-bound partners. It is practically identical for Pcs from plants and green algae, but is quite different for Pc from ferns. Here we report on a laser flash kinetic analysis of PSI reduction by Pc from various eukaryotic and prokaryotic organisms. The reaction of fern Pc with fern PSI fits a two-step kinetic model, consisting of complex formation and electron transfer, whereas other plant systems exhibit a mechanism that requires an additional intracomplex rearrangement step. The fern Pc interacts inefficiently with spinach PSI, showing no detectable complex formation. This can be explained by assuming that the unusual surface charge distribution of fern Pc impairs the interaction. Fern PSI behaves in a similar way as spinach PSI in reaction with other Pcs. The reactivity of fern Pc towards several soluble c-type cytochromes, including cytochrome f, has been analysed by flavin-photosensitized laser flash photolysis, demonstrating that the specific surface motifs for the interaction with cytochrome f are conserved in fern Pc. | en_US |
dc.description.sponsorship | Research work was supported by the Spanish Ministry of Science and Technology (MCYT, Grant BMC2003-0458), and Andalusian Government (PAI, CVI-0198). C. E. Lowe acknowledges the financial support provided through the European Community's Human Potential Programme under contracts FMRX-CT98-0218 (Haemworks) and HPRN-CT-1999-00095 (Transient). M. Ubbink acknowledges financial support from the Netherlands Organization for Scientific Research, grant 700.52.425. | en_US |
dc.format.extent | 7450 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | John Wiley & Sons | en_US |
dc.rights | closedAccess | en_US |
dc.subject | Dryopteris | en_US |
dc.subject | Fern | en_US |
dc.subject | Nephrolepsis | en_US |
dc.subject | Photosystem I | en_US |
dc.subject | Plastocyanin | en_US |
dc.title | Functional characterization of the evolutionarily divergent fern plastocyanin | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1111/j.0014-2956.2004.04283.x | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1111/j.0014-2956.2004.04283.x | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
Aparece en las colecciones: | (IBVF) Artículos |
CORE Recommender
SCOPUSTM
Citations
5
checked on 28-abr-2024
WEB OF SCIENCETM
Citations
6
checked on 28-feb-2024
Page view(s)
343
checked on 05-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.