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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/8276
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dc.contributor.authorLavarino, Cinzia-
dc.contributor.authorMackintosh, Carlos-
dc.contributor.authorRíos, José-
dc.contributor.authorRodríguez, Eva-
dc.contributor.authorTorres, Carmen de-
dc.contributor.authorÁlava, Enrique de-
dc.contributor.authorMora, Jaume-
dc.date.accessioned2008-11-06T13:37:55Z-
dc.date.available2008-11-06T13:37:55Z-
dc.date.issued2008-08-13-
dc.identifier.citationBMC Medical Genomics 1: 36 (2008)en_US
dc.identifier.issn1755-8794-
dc.identifier.urihttp://hdl.handle.net/10261/8276-
dc.descriptionThis is an Open Access article distributed under the terms of the Creative Commons Attribution License.-- et al.-
dc.description.abstract[Background]: Neuroblastic tumours (NBTs) represent a heterogeneous spectrum of neoplastic diseases associated with multiple genetic alterations. Structural and numerical chromosomal changes are frequent and are predictive parameters of NBTs outcome. We performed a comparative analysis of the biological entities constituted by NBTs with different ploidy status. [Methods]: Gene expression profiling of 49 diagnostic primary NBTs with ploidy data was performed using oligonucleotide microarray. Further analyses using Quantitative Real-Time Polymerase Chain Reaction (Q-PCR); array-Comparative Genomic Hybridization (aCGH); and Fluorescent in situ Hybridization (FISH) were performed to investigate the correlation between aneuploidy, chromosomal changes and gene expression profiles. [Results]: Gene expression profiling of 49 primary near-triploid and near-diploid/tetraploid NBTs revealed distinct expression profiles associated with each NBT subgroup. A statistically significant portion of genes mapped to 1p36 (P = 0.01) and 17p13-q21 (P < 0.0001), described as recurrently altered in NBTs. Over 90% of these genes showed higher expression in near-triploid NBTs and the majority are involved in cell differentiation pathways. Specific chromosomal abnormalities observed in NBTs, 1p loss, 17q and whole chromosome 17 gains, were reflected in the gene expression profiles. Comparison between gene copy number and expression levels suggests that differential expression might be only partly dependent on gene copy number. Intratumoural clonal heterogeneity was observed in all NBTs, with marked interclonal variability in near-diploid/tetraploid tumours. [Conclusion]: NBTs with different cellular DNA content display distinct transcriptional profiles with a significant portion of differentially expressed genes mapping to specific chromosomal regions known to be associated with outcome. Furthermore, our results demonstrate that these specific genetic abnormalities are highly heterogeneous in all NBTs, and suggest that NBTs with different ploidy status may result from different mechanisms of aneuploidy driving tumourigenesis.en_US
dc.description.sponsorshipThis work was supported by: Career Development Award 2001 (to J. M.) from the American Society of Clinical Oncology (ASCO) and grants from the Spanish Ministry of Health (Instituto de Salud Carlos III, Fondo de Investigación Sanitaria, 2007; PI070286) (CL) and Spanish Society against Cancer (Asociación Española Contra el Cáncer, 2007) (JM and CL). The Developmental tumour biology laboratory, Hospital Sant Joan de Déu in Barcelona, is additionally supported by the Catalan government (AGAUR, Generalitat de Catalunya, 2005SGR00605; 2006FI00404), and the donation from Margarita del Pozo Fund. Supported in part by the National Cancer Institute grant CA106450 (NKC and WG), The Robert Steel Foundation (NKC), Hope Street Kids (NKC), and Katie's Find A Cure Fund (NKC) and the Government of Castilla y León (EdA).en_US
dc.format.extent604160 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofPublisher's version-
dc.rightsopenAccessen_US
dc.subjectGenes mappingen_US
dc.subjectNeuroblastic tumoursen_US
dc.subjectAbnormal chromosomal regionsen_US
dc.titleDifferential expression of genes mapping to recurrently abnormal chromosomal regions characterize neuroblastic tumours with distinct ploidy statusen_US
dc.typeartículoen_US
dc.identifier.doi10.1186/1755-8794-1-36-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1186/1755-8794-1-36en_US
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderGeneralitat de Catalunya-
dc.contributor.funderAsociación Española Contra el Cáncer-
dc.contributor.funderAmerican Society of Clinical Oncology-
dc.contributor.funderMinisterio de Sanidad y Política Social (España)-
dc.relation.csicSí-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004587es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100002809es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100006293es_ES
dc.identifier.pmid18700951-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.openairetypeartículo-
item.fulltextWith Fulltext-
item.languageiso639-1en-
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