Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/79103
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Dynamics and environment of mitochondrial water as detected by 1H NMR |
Autor: | López-Beltrán, Emilio A.; Maté, María J. CSIC; Cerdán, Sebastián CSIC ORCID | Fecha de publicación: | 1996 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 271(18): 10648-10653 (1996) | Resumen: | The dynamics and environment of water in suspensions of isolated rat liver mitochondria have been investigated by 1H NMR. NMR longitudinal and transversal relaxation times (T1 and T2) were measured in the resuspension medium (2.65 s and 44.57 ms) and in mitochondrial suspensions (1.74 s and 23.14 ms), respectively. Results showed monoexponential relaxation in both cases, suggesting a fast water exchange across the inner mitochondrial membrane. Ferromagnetically induced shift of the extramitochondrial water with nonpermeant ferromagnetic particles revealed no detectable water signal from the intramitochondrial compartment, confirming the fast exchange case. Simulations on a two-compartment model indicated that the intramitochondrial water residence time has an upper limit of approximately 100 μs. Calculated intramitochondrial relaxation times revealed that the intramitochondrial environment has an apparent viscosity 30 times larger than the resuspension medium and 15 times larger than the cytosol of erythrocytes. The higher apparent viscosity of the mitochondrial matrix could account for reductions of more than one order of magnitude in the diffusion coefficient of water and other substrates, limitations in the rate of enzymatic reactions which are diffusion controlled and a more favorable formation of multienzyme complexes. | URI: | http://hdl.handle.net/10261/79103 | DOI: | 10.1074/jbc.271.18.10648 | Identificadores: | doi: 10.1074/jbc.271.18.10648 issn: 0021-9258 e-issn: 1083-351X |
Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
43
checked on 11-may-2024
WEB OF SCIENCETM
Citations
44
checked on 22-feb-2024
Page view(s)
307
checked on 19-may-2024
Download(s)
101
checked on 19-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.