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Title

Differential expression of a gene highly homologous to c-ras during the development of the brine shrimp Artemia

AuthorsDíaz-Guerra, Margarita; Quintanilla, Miguel ; Palmero, Ignacio ; Sastre, Leandro ; Renart, Jaime
Issue Date1989
PublisherElsevier
CitationBiochemical and Biophysical Research Communications 162(2): 802-808 (1989)
AbstractWe report the identification of p21 ras and a cDNA coding for it in the crustacean Artemia. The monoclonal antibody Y13-259 immunoprecipitates a polypeptide of 21.5 kDa in 24 hr-old larvae. The homology of p21 ras with the Drosophila melanogaster and the mammalian p21s is in the order of 75-80%. mRNA (of 1.2 kb in length) is already present in encysted gastrulae, and the levels increase, reaching a maximum before emergence. On the contrary, both the amount of p21 ras and its GTP-binding activity are low prior to emergence and rises afterwards. These results suggest that p21 ras expression is regulated post-transcriptionally, and that its function(s) is needed for post-hatching events, the more likely being the resumption of cell proliferation.
URIhttp://hdl.handle.net/10261/78778
DOI10.1016/0006-291X(89)92381-4
Identifiersdoi: 10.1016/0006-291X(89)92381-4
issn: 0006-291X
e-issn: 1090-2104
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