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dc.contributor.author | Peña, Alejandro | - |
dc.contributor.author | Ripoll-Rozada, Jorge | - |
dc.contributor.author | Zunzunegui, Sandra | - |
dc.contributor.author | Cabezón, Elena | - |
dc.contributor.author | Cruz, Fernando de la | - |
dc.contributor.author | Arechaga, Ignacio | - |
dc.date.accessioned | 2013-06-27T11:59:38Z | - |
dc.date.available | 2013-06-27T11:59:38Z | - |
dc.date.issued | 2011 | - |
dc.identifier | doi: 10.1074/jbc.M110.208942 | - |
dc.identifier | issn: 0021-9258 | - |
dc.identifier | e-issn: 1083-351X | - |
dc.identifier.citation | Journal of Biological Chemistry 286(19): 17376-17382 (2011) | - |
dc.identifier.uri | http://hdl.handle.net/10261/78728 | - |
dc.description.abstract | Type IV secretion systems (T4SS) mediate the transfer of DNA and protein substrates to target cells. TrwK, encoded by the conjugative plasmid R388, is a member of the VirB4 family, comprising the largest and most conserved proteins of T4SS. In a previous work we demonstrated that TrwK is able to hydrolyze ATP. Here, based on the structural homology of VirB4 proteins with the DNA-pumping ATPase TrwB coupling protein, we generated a series of variants of TrwK where fragments of the C-terminal domain were sequentially truncated. Surprisingly, the in vitro ATPase activity of these TrwK variants was much higher than that of the wild-type enzyme. Moreover, addition of a synthetic peptide containing the amino acid residues comprising this C-terminal region resulted in the specific inhibition of the TrwK variants lacking such domain. These results indicate that the C-terminal end of TrwK plays an important regulatory role in the functioning of the T4SS. | - |
dc.description.sponsorship | This work was supported by the Ministerio de Ciencia e Innovación (MCINN, Spain) Grants BFU2008-00806 and BFU2008-00995/BMC, RD06/0008/1012 from Instituto de Salud Carlos III (Spain) and LSHM-CT-2005_019023 from the European VI Framework Program. | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.rights | closedAccess | - |
dc.title | Autoinhibitory regulation of TrwK, an essential VirB4 ATPase in type IV secretion systems | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1074/jbc.M110.208942 | - |
dc.date.updated | 2013-06-27T11:59:38Z | - |
dc.description.version | Peer Reviewed | - |
dc.identifier.pmid | 21454654 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
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