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Título: | Synthase III-dependent chitin is bound to different acceptors depending on location on the cell wall of budding yeast |
Autor: | Cabib, Enrico; Durán, Ángel CSIC | Fecha de publicación: | 2005 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 280(10): 9170-9179 (2005) | Resumen: | In yeast, chitin is laid down at three locations: a ring at the mother-bud neck, the primary septum and, after cytokinesis, the cell wall of the daughter cell. Some of the chitin is free and the remainder attached to β(1–3)glucan or β(1–6)glucan. We recently reported that the chitin ring contributes to the prevention of growth at the mother-bud neck and hypothesized that this inhibition is achieved by a preferential binding of chitin to β(1–3)glucan at that site. Here, we devised a novel strategy for the analysis of chitin cross-links in [14C]glucosamine-labeled cell walls, involving solubilization in water of alkali-treated walls by carboxymethylation. Intact cell walls or their digestion products with β(1–3)glucanase or β(1–6)glucanase were carboxymethylated and fractionated on size columns, and the percentage of chitin bound to different polysaccharides was calculated. Chitin dispersed in the wall was labeled in maturing unbudded cells and that of the ring in early budding cells. The former was mostly attached to β(1–6)glucan and the latter to β(1–3)glucan. This confirmed our hypothesis and indicated that the cell has mechanisms to attach chitin, a water-insoluble substance, synthesized here through chitin synthase III, to different acceptors, depending on location. In contrast, most of the chitin synthase II-dependent chitin of the primary septum was free, with the remainder linked to β(1–3)glucan. | URI: | http://hdl.handle.net/10261/67161 | DOI: | 10.1074/jbc.M414005200 | Identificadores: | doi: 10.1074/jbc.M414005200 issn: 0021-9258 e-issn: 1083-351X |
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