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Título: | Schizosaccharomyces pombe ehs1p is involved in maintaining cell wall integrity and in calcium uptake |
Autor: | Carnero, Elena CSIC; Ribas, Juan Carlos CSIC ORCID; García, Blanca; Durán, Ángel CSIC; Sánchez, Yolanda CSIC | Fecha de publicación: | 2000 | Editor: | Springer Nature | Citación: | Molecular and General Genetics 264(1-2): 173-183 (2000) | Resumen: | The Schizosaccharomyces pombe mutant ehs1-1 mutant was isolated on the basis of its hypersensitivity to Echinocandin and Calcofluor White, which inhibit cell wall synthesis. The mutant shows a thermosensitive growth phenotype that is suppressed in the presence of an osmotic stabiliser. The mutant also showed other cell wall-associated phenotypes, such as enhanced sensitivity to enzymatic cell wall degradation and an imbalance in polysaccharide synthesis. The ehs1+ gene encodes a predicted integral membrane protein that is 30% identical to Saccharomyces cerevisiae Midlp, a protein that has been proposed to form part of a calcium channel. As expected for such a function, we found that ehs1+ is involved in intracellular Ca2+ accumulation. High external Ca2+ concentrations suppressed all phenotypes associated with the ehs1 null mutation, suggesting that the cell integrity defects of ehs1 mutants result from inadequate levels of calcium in the cell. We observed a genetic relationship between ehs1+ and the protein kinase C homologue pck2+. pck2+ suppressed all phenotypes of ehs1-1 mutant cells. Overproduction of pck2p is deleterious to wild-type cells, increasing 1,3-β-D-glucan synthase activity and promoting accumulation of extremely high levels of Ca2+. The lethality associated with pck2p, the increase in 1,3-β-D-glucan synthase production and the strong Ca2+ accumulation are all dependent on the presence of ehs1p. Our results suggest that in fission yeast ehs1p forms part of a calcium channel that is involved in the cell wall integrity pathway that includes the kinase pck2p. | URI: | http://hdl.handle.net/10261/66945 | Identificadores: | issn: 0026-8925 e-issn: 1432-1874 |
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