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Título: | Motorneurons Require Cysteine String Protein-α to Maintain the Readily Releasable Vesicular Pool and Synaptic Vesicle Recycling |
Autor: | Rozas, José Luis CSIC; Gómez-Sánchez, L.; Mircheski, Josif; Linares-Clemente, P.; Nieto-González, J. CSIC ORCID; Vázquez, M. Eugenio; Luján, Rafael; Fernández-Chacón, Rafael CSIC ORCID | Fecha de publicación: | 2012 | Editor: | Elsevier | Citación: | Neuron 74(1): 151-165 (2012) | Resumen: | Cysteine string protein-α (CSP-α) is a synaptic vesicle protein that prevents activity-dependent neurodegeneration by poorly understood mechanisms. We have studied the synaptic vesicle cycle at the motor nerve terminals of CSP-α knock-out mice expressing the synaptopHluorin transgene. Mutant nerve terminals fail to sustain prolonged release and the number of vesicles available to be released decreases. Strikingly, the SNARE protein SNAP-25 is dramatically reduced. In addition, endocytosis during the stimulus fails to maintain the size of the recycling synaptic vesicle pool during prolonged stimulation. Upon depolarization, the styryl dye FM 2-10 becomes trapped and poorly releasable. Consistently with the functional results, electron microscopy analysis revealed characteristic features of impaired synaptic vesicle recycling. The unexpected defect in vesicle recycling in CSP-α knock-out mice provides insights into understanding molecular mechanisms of degeneration in motor nerve terminals. Cysteine string protein-α (CSP-α) is a synaptic vesicle protein that prevents activity-dependent neurodegeneration. Rozas et al. have used synaptopHluorin imaging and electrophysiology to find alterations in synaptic vesicle priming and recycling at motor nerve terminals of CSP-α KO mice. © 2012 Elsevier Inc. | URI: | http://hdl.handle.net/10261/62811 | DOI: | 10.1016/j.neuron.2012.02.019 | Identificadores: | doi: 10.1016/j.neuron.2012.02.019 issn: 0896-6273 |
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