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http://hdl.handle.net/10261/47336
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Jiménez, Alberto | - |
dc.contributor.author | Oko, Richard | - |
dc.contributor.author | Gustafsson, Jan-Åke | - |
dc.contributor.author | Spyrou, Giannis | - |
dc.contributor.author | Pelto-Huikko, Markku | - |
dc.contributor.author | Miranda-Vizuete, Antonio | - |
dc.date.accessioned | 2012-03-22T10:54:32Z | - |
dc.date.available | 2012-03-22T10:54:32Z | - |
dc.date.issued | 2002-08 | - |
dc.identifier.citation | Human Molecular Genetics 8(8): 710-718 (2002) | es_ES |
dc.identifier.issn | 1360-9947 | - |
dc.identifier.other | PMID: 12149401 | - |
dc.identifier.uri | http://hdl.handle.net/10261/47336 | - |
dc.description | 31 páginas, 5 figuras. | es_ES |
dc.description.abstract | Thioredoxins are proteins that participate in different cellular processes via redox-mediated reactions. For humans, we have recently described two novel members of this family that display a male germ cell specific expression pattern, named spermatid specific thioredoxin (Sptrx-1 and Sptrx-2 respectively). We report here the cloning and characterization of the mouse Sptrx-1 gene and protein, which are similar to those described for the human orthologue. The mouse Sptrx-1 open reading frame encodes for a protein of 462 aa composed of an N-terminal repetitive domain of a 15 residue motif followed by a C-terminal domain typical of thioredoxins. The mouse Sptrx-1 gene sequence is interrupted by only one intron of 525 bp located in the 5′-UTR, and using fluorescence in-situ hybridization we have mapped its chromosomal location to 17E1.2–1.3. Northern blot analysis identified the testis as the only tissue expressing mouse Sptrx-1 mRNA, and by in-situ hybridization we found a strong labelling in the testicular seminiferous tubules, mostly in the round spermatids. Affinity purified antibodies against human Sptrx-1 crossreacted well with the mouse protein confirming its expression in seminiferous tubules at the later stages of spermiogenesis. Recombinant mouse Sptrx-1 displayed protein disulphide reducing activity in an enzymatic assay coupled to NADPH and thioredoxin reductase. The availability of the mouse Sptrx-1 gene sequence is the first step towards the generation of knock-out mice, whose characterization will provide significant information regarding the in-vivo function of Sptrx-1 and its possible implication in several sperm anomalies. | es_ES |
dc.description.sponsorship | This work was supported by grants from the Swedish Medical Research Council (Projects 03P-14096-01A, 03X-14041-01A and 13X-10370), the Åke Wibergs Stiftelse, the Karolinska Institutet, the Medical Research Fund of Tampere University Hospital and NSERC (to R.O.). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Fibrous sheath | es_ES |
dc.subject | Sperm | es_ES |
dc.subject | Testis | es_ES |
dc.subject | Thioredoxin | es_ES |
dc.title | Cloning, expression and characterization of mouse spermatid specific thioredoxin-1 gene and protein | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1093/molehr/8.8.710 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1093/molehr/8.8.710 | es_ES |
dc.identifier.e-issn | 1460-2407 | - |
dc.identifier.pmid | 12149401 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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