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dc.contributor.authorCaraux, Anouk-
dc.contributor.authorPaiva, Bruno-
dc.contributor.authorOrfao, Alberto-
dc.contributor.authorPérez-Andrés, Martin-
dc.identifier.citationHaematologica 95(6): 1016-1020 (2010)es_ES
dc.description5 páginas, 2 figuras.es_ES
dc.description.abstractGeneration of B and plasma cells involves several organs with a necessary cell trafficking between them. A detailed phenotypic characterization of four circulating B-cell subsets (immature-, naïve-, memory- B-lymphocytes and plasma cells) of 106 healthy adults was realized by multiparametric flow cytometry. We show that CD10, CD27 and CD38 is the minimal combination of subsetting markers allowing unequivocal identification of immature (CD10+CD27−CD38+, 6±6 cells/μL), naïve (CD10−CD27−CD38−, 125±90 cells/μL), memory B lymphocytes (CD10−CD27+CD38−, 58±42 cells/μL), and plasma cells (CD10−CD27++CD38++, 2.1±2.1 cells/μL) within circulating CD19+ cells. From these four subsets, only memory B lymphocytes and plasma cells decreased with age, both in relative and absolute counts. Circulating plasma cells split into CD138− (57±12%) and CD138+ (43±12%) cells, the latter displaying a more mature phenotypic profile: absence of surface immunoglobulin, lower CD45 positivity and higher amounts of cytoplasmic immunoglobulin, CD38 and CD27. Unlike B lymphocytes, both populations of plasma cells are KI-67+ and show weak CXCR4 expression.es_ES
dc.description.sponsorshipThis work was supported by grants from the Ligue Nationale Contre le Cancer (équipe labellisée 2009), Paris, France, from INCA (n. R07001FN), the Fondo de Investigación Sanitaria, Ministerio de Ciencia e Innovación (FIS 06-0824), Madrid, Spain, Gerencia Regional de Salud de Castilla y León (GRS206/A/08),Valladolid, Spain, the AYUDA PARA LA FINANCIACIÓN DE LOS PROGRAMAS DE ACTIVIDAD INVESTIGADORA DE LOS GRUPOS DE INVESTIGACIÓN DE EXCELENCIA DE CASTILLA Y LEÓN (EDU/894/2009, GR37), Junta de Castilla y León, Valladolid, the Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación (RTICC RD06/0020/0035), Madrid, Spain, and from MSCNET European strep (n. E06005FF) Cancer Centers Research Network.-
dc.publisherFerrata Storti Foundationes_ES
dc.subjectHealthy donorses_ES
dc.subjectBone marrowes_ES
dc.subjectPlasma cellses_ES
dc.subjectPeripheral bloodes_ES
dc.subjectDendritic cellses_ES
dc.titleCirculating human b and plasma cells. Age-associated changes in counts and detailed characterization of circulating normal CD138– and CD138+ plasma cellses_ES
dc.description.peerreviewedPeer reviewedes_ES
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