Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/43726
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | DNA barcoding insect–host plant associations |
Autor: | Jurado-Rivera, José A. CSIC ORCID; Vogler, Alfried P.; Reid, Chris A. M.; Petitpierre, Eduard CSIC; Gómez-Zurita, Jesús CSIC ORCID | Palabras clave: | Herbivory Host plant Molecular identification Coevolution trnL cpDNA |
Fecha de publicación: | feb-2009 | Editor: | Royal Society (Great Britain) | Citación: | Proceedings B: Biological Sciences 276(1657): 639-648 (2009) | Resumen: | Short-sequence fragments (‘DNA barcodes’) used widely for plant identification and inventorying remain to be applied to complex biological problems. Host–herbivore interactions are fundamental to coevolutionary relationships of a large proportion of species on the Earth, but their study is frequently hampered by limited or unreliable host records. Here we demonstrate that DNA barcodes can greatly improve this situation as they (i) provide a secure identification of host plant species and (ii) establish the authenticity of the trophic association. Host plants of leaf beetles (subfamily Chrysomelinae) from Australia were identified using the chloroplast trnL(UAA) intron as barcode amplified from beetle DNA extracts. Sequence similarity and phylogenetic analyses provided precise identifications of each host species at tribal, generic and specific levels, depending on the available database coverage in various plant lineages. The 76 species of Chrysomelinae included—more than 10 per cent of the known Australian fauna—feed on 13 plant families, with preference for Australian radiations of Myrtaceae (eucalypts) and Fabaceae (acacias). Phylogenetic analysis of beetles shows general conservation of host association but with rare host shifts between distant plant lineages, including a few cases where barcodes supported two phylogenetically distant host plants. The study demonstrates that plant barcoding is already feasible with the current publicly available data. By sequencing plant barcodes directly from DNA extractions made from herbivorous beetles, strong physical evidence for the host association is provided. Thus, molecular identification using short DNA fragments brings together the detection of species and the analysis of their interactions. | Descripción: | 11 páginas, 3 figuras, 2 tablas. | Versión del editor: | http://dx.doi.org/10.1098/rspb.2008.1264 | URI: | http://hdl.handle.net/10261/43726 | DOI: | 10.1098/rspb.2008.1264 | E-ISSN: | 1471-2954 |
Aparece en las colecciones: | (IBE) Artículos (IMEDEA) Artículos |
Mostrar el registro completo
CORE Recommender
PubMed Central
Citations
58
checked on 30-abr-2024
SCOPUSTM
Citations
190
checked on 07-may-2024
WEB OF SCIENCETM
Citations
170
checked on 24-feb-2024
Page view(s)
413
checked on 13-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.