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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Blanco, Luis | - |
dc.contributor.author | Lázaro, José M. | - |
dc.contributor.author | Vega, Miguel de | - |
dc.contributor.author | Bonnin, Ana | - |
dc.contributor.author | Salas, Margarita | - |
dc.date.accessioned | 2011-09-20T10:01:31Z | - |
dc.date.available | 2011-09-20T10:01:31Z | - |
dc.date.issued | 1994-12-06 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the USA 91(25): 12198-12202 (1994) | es_ES |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10261/39725 | - |
dc.description | This work is dedicated to the memory of Severo Ochoa. | es_ES |
dc.description.abstract | By using appropriate amounts of four bacteriophage phi 29 DNA replication proteins--terminal protein, DNA polymerase, protein p6 (double-stranded DNA-binding protein), and protein p5 (single-stranded DNA-binding protein)--it has been possible to amplify limited amounts of the 19,285-bp-long phi 29 DNA molecule by three orders of magnitude after 1 hr of incubation at 30 degrees C. Moreover, the quality of the amplified material was demonstrated by transfection experiments, in which infectivity of the synthetic (amplified) phi 29 DNA, measured as the ability to produce phage particles, was identical to that of the natural phi 29 DNA obtained from virions. The results presented in this paper establish some of the requisites for the development of isothermal DNA amplification strategies based on the bacteriophage phi 29 DNA replication machinery that are suitable for the amplification of very large (> 70 kb) segments of DNA. | es_ES |
dc.description.sponsorship | This investigation was aided by Research Grant 5R01 GM27242-15 from the National Institutes of Health, by Grant PB90-0091 from Direcci6n General de Investigaci6n Cientifica y Tdcnica, by Grant BIOT CT 91-0268 from the European Economic Community, and by an Institutional grant from Fundaci6n Ram6n Areces. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | National Academy of Sciences (U.S.) | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Terminal protein-primed DNA amplification | es_ES |
dc.type | artículo | es_ES |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://www.pnas.org/content/91/25/12198.short | es_ES |
dc.identifier.e-issn | 1091-6490 | - |
dc.contributor.funder | National Institutes of Health (US) | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Fundación Ramón Areces | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100000002 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100008054 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
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12198.full.pdf | 1,25 MB | Adobe PDF | Visualizar/Abrir |
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