3B1 binds the bottom of the 3Dpol palm also in solution [Dataset]

Ferrer-Orta, Cristina; Ferrero, Diego; Verdaguer, Núria

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Título:	3B1 binds the bottom of the 3Dpol palm also in solution [Dataset]
Autor:	Ferrer-Orta, Cristina CSIC ORCID ; Ferrero, Diego CSIC ORCID ; Verdaguer, Núria CSIC ORCID
Palabras clave:	Structural biology approaches Mouth disease virus div >< p Different functions within Almost symmetric way Linked immunosorbent assays Play essential roles Concentrate viral rna Two uridine molecules pol Pol Another critical interaction Animal pathogen foot Region ii presenting Higher binding affinity Binding protein responsible 3b1 complex Dependent rna polymerase 3b1 region ii Pprimer protein 3b Elisa assays show Viral rna Protein primer Region ii Assays show Small protein Replication complex Highest affinity Essential component Functional binding Fmdv 3b1 Replication requires Replication occurs Replication machinery Picornavirus replication Membranous compartments Identical copies Host factors Basic residues Based pull Also participate Also catalysed 3b uridylylation
Fecha de publicación:	1-may-2023
Editor:	Figshare
Citación:	Ferrer-Orta, Cristina; Ferrero, Diego; Verdaguer, Núria; 2023; 3B1 binds the bottom of the 3Dpol palm also in solution [Dataset]; Figshare; https://doi.org/10.1371/journal.ppat.1011373.g004
Resumen:	(A) Enzyme-Linked Immunosorbent Assay (ELISA) to compare the binding affinities of 3Dpol I and 3Dpol II binding sites of 3B1, determined in the X-ray structure. A multi-well plate was coated with 10 μg/ml of 3Dpol and its interaction to GST-3B1 WT was measured at increasing concentrations (from 0.5μg/ml to 20 μg/ml) and compared with mutants: GST-3B1(P6S/R9A), GST-3B1(R16A/L19S), GST-3B1(P6S/R9A/R16A/L19S) and GST. (B) ELISA assay to compare the affinities of 3B1, 3B2 and 3B3 for 3Dpol. Data were obtained from three independent experiments and standard deviations are reported.
Versión del editor:	https://doi.org/10.1371/journal.ppat.1011373.g004
URI:	http://hdl.handle.net/10261/351206
DOI:	10.1371/journal.ppat.1011373.g004
Referencias:	Ferrer-Orta, Cristina; Ferrero, Diego; Verdaguer, Núria. Dual role of the foot-and-mouth disease virus 3B1 protein in the replication complex: As protein primer and as an essential component to recruit 3Dpol to membranas. https://doi.org/10.1371/journal.ppat.1011373 . http://hdl.handle.net/10261/335548
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