Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/346458
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Generation of subtype-specific neurons from postnatal astroglia of the mouse cerebral cortex |
Autor: | Heinrich, Christophe; Gascón, Sergio CSIC ORCID; Masserdotti, Giacomo; Lepier, Alexandra; Sanchez, Rodrigo; Simon-Ebert, Tatiana; Schroeder, Timm; Götz, Magdalena; Berninger, Benedikt | Fecha de publicación: | feb-2011 | Editor: | Nature Publishing Group | Citación: | Nature Protocols | Resumen: | Instructing glial cells to generate neurons may prove to be a strategy to replace neurons that have degenerated. Here, we describe a robust protocol for the efficient in vitro conversion of postnatal astroglia from the mouse cerebral cortex into functional, synapse-forming neurons. This protocol involves two steps: (i) expansion of astroglial cells (7 d) and (ii) astroglia-to-neuron conversion induced by persistent and strong retroviral expression of Neurog2 (encoding neurogenin-2) or Mash1 (also referred to as achaete-scute complex homolog 1 or Ascl1) and/or distal-less homeobox 2 (Dlx2) for generation of glutamatergic or GABAergic neurons, respectively (7-21 d for different degrees of maturity). Our protocol of astroglia-to-neuron conversion by a single neurogenic transcription factor provides a stringent experimental system to study the specification of a selective neuronal subtype, thus offering an alternative to the use of embryonic or neural stem cells. Moreover, it can be a useful model for studies of lineage conversion from non-neuronal cells, with potential for brain regenerative medicine. | Versión del editor: | https://www.nature.com/articles/nprot.2010.188 | URI: | http://hdl.handle.net/10261/346458 | DOI: | 10.1038/nprot.2010.188 | ISSN: | 17542189 |
Aparece en las colecciones: | (IC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
nprot.2010.188.pdf | 2,29 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
70
checked on 07-may-2024
SCOPUSTM
Citations
112
checked on 08-may-2024
WEB OF SCIENCETM
Citations
105
checked on 29-feb-2024
Page view(s)
8
checked on 12-may-2024
Download(s)
5
checked on 12-may-2024