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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Pionnier, Nicolas | es_ES |
dc.contributor.author | Falcó, Alberto | es_ES |
dc.contributor.author | Miest, Joanna | es_ES |
dc.contributor.author | Frost, Patrick | es_ES |
dc.contributor.author | Irnazarow, Ilgiz | es_ES |
dc.contributor.author | Shrive, Annette K. | es_ES |
dc.contributor.author | Hoole, David | es_ES |
dc.date.accessioned | 2024-02-05T15:53:11Z | - |
dc.date.available | 2024-02-05T15:53:11Z | - |
dc.date.issued | 2013-03 | - |
dc.identifier.citation | Fish & Shellfish Immunology 34(3): 819-831 (2013) | es_ES |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | http://hdl.handle.net/10261/345439 | - |
dc.description.abstract | The effect of β-glucans as feed additive on the profile of C-reactive protein (CRP) and complement acute phase responses was studied in common carp Cyprinus carpio after exposition to a bacterial infection with Aeromonas salmonicida. Carp were orally administered with β-glucan (MacroGard®) for 14 days with a daily β-glucan intake of 6 mg per kg body weight. Fish were then intraperitoneally injected with either PBS or 1 × 108 bacteria per fish and sampled at time 0, 6, 12, 24, 48, 72, 96 and 120 h post-injection (p.i.) for serum and head kidney, liver and mid-gut tissues. CRP levels and complement activity were determined in the serum samples whilst the gene expression profiles of CRP and complement related genes (crp1, crp2, c1r/s, bf/c2, c3 and masp2) were analysed in the tissues by quantitative PCR. Results obtained showed that oral administration of β-glucan for 14 days significantly increased serum CRP levels up to 2 fold and serum alternative complement activity (ACP) up to 35 fold. The bacterial infection on its own (i.e. not combined with a β-glucan feeding) did have significant effects on complement response whilst CRP was not detectably induced during the carp acute phase reaction. However, the combination of the infection and the β-glucan feeding did show significant effects on both CRP and complement profiles with higher serum CRP levels and serum ACP activity in the β-glucan fed fish than in the control fed fish. In addition, a distinct organ and time dependent expression profile pattern was detected for all the selected genes: a peak of gene expression first occurred in the head kidney tissue (6 h p.i. or 12 h p.i.), then an up-regulation in the liver several hours later (24 h p.i.) and finally up- or down-regulations in the mid-gut at 24 h p.i. and 72 h p.i. In conclusion, the results of this study suggest that MacroGard® stimulated CRP and complement responses to A. salmonicida infection in common carp. | es_ES |
dc.description.sponsorship | The research leading to these results has received funding from the European Community's Seventh Framework Programme ([FP7/2007-2013] under grant agreement no PITN-GA-2008-214505). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/214505 | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Beta-glucan | es_ES |
dc.subject | C-reactive protein | es_ES |
dc.subject | Complement | es_ES |
dc.subject | Aeromonas salmonicida | es_ES |
dc.subject | Cyprinus carpio | es_ES |
dc.title | Dietary β-glucan stimulate complement and C-reactive protein acute phase responses in common carp (Cyprinus carpio) during an Aeromonas salmonicida infection | es_ES |
dc.type | artículo | es_ES |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.fsi.2012.12.017 | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.relation.csic | No | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
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