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dc.contributor.author | Łapińska, Urszula | es_ES |
dc.contributor.author | Glover, Georgina | es_ES |
dc.contributor.author | Kahveci, Zehra | es_ES |
dc.contributor.author | Irwin, Nicholas A. T. | es_ES |
dc.contributor.author | Milner, David S. | es_ES |
dc.contributor.author | Tourte, Maxime | es_ES |
dc.contributor.author | Albers, Sonja-Verena | es_ES |
dc.contributor.author | Santoro, Alyson E. | es_ES |
dc.contributor.author | Richards, Thomas A. | es_ES |
dc.contributor.author | Pagliara, Stefano | es_ES |
dc.date.accessioned | 2024-01-31T10:34:20Z | - |
dc.date.available | 2024-01-31T10:34:20Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | PLoS Biology 21(4): e3002048 (2023) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/344599 | - |
dc.description.abstract | One of the deepest branches in the tree of life separates the Archaea from the Bacteria. These prokaryotic groups have distinct cellular systems including fundamentally different phospholipid membrane bilayers. This dichotomy has been termed the lipid divide and possibly bestows different biophysical and biochemical characteristics on each cell type. Classic experiments suggest that bacterial membranes (formed from lipids extracted from Escherichia coli, for example) show permeability to key metabolites comparable to archaeal membranes (formed from lipids extracted from Halobacterium salinarum), yet systematic analyses based on direct measurements of membrane permeability are absent. Here, we develop a new approach for assessing the membrane permeability of approximately 10 μm unilamellar vesicles, consisting of an aqueous medium enclosed by a single lipid bilayer. Comparing the permeability of 18 metabolites demonstrates that diether glycerol-1-phosphate lipids with methyl branches, often the most abundant membrane lipids of sampled archaea, are permeable to a wide range of compounds useful for core metabolic networks, including amino acids, sugars, and nucleobases. Permeability is significantly lower in diester glycerol-3-phosphate lipids without methyl branches, the common building block of bacterial membranes. To identify the membrane characteristics that determine permeability, we use this experimental platform to test a variety of lipid forms bearing a diversity of intermediate characteristics. We found that increased membrane permeability is dependent on both the methyl branches on the lipid tails and the ether bond between the tails and the head group, both of which are present on the archaeal phospholipids. These permeability differences must have had profound effects on the cell physiology and proteome evolution of early prokaryotic forms. To explore this further, we compare the abundance and distribution of transmembrane transporter-encoding protein families present on genomes sampled from across the prokaryotic tree of life. These data demonstrate that archaea tend to have a reduced repertoire of transporter gene families, consistent with increased membrane permeation. These results demonstrate that the lipid divide demarcates a clear difference in permeability function with implications for understanding some of the earliest transitions in cell origins and evolution. | es_ES |
dc.description.sponsorship | This work was supported by the Gordon and Betty Moore Foundation Marine Microbiology Initiative (GBMF5514 to TAR., SP and AES), the Biotechnology and Biological Sciences Research Council (BB/V008021/1 to SP and UL), the H2020 Marie Skłodowska-Curie Actions (H2020-MSCA-ITN-2015-675752 to SP and TAR), the Volkswagen foundation (Life? Az 96727 to MT and SVA) and Merton College, University of Oxford (NATI). | es_ES |
dc.format | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/675752 | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.relation.isbasedon | The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1371/journal.pbio.3002048 | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Systematic comparison of unilamellar vesicles reveals that archaeal core lipid membranes are more permeable than bacterial membranes | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1371/journal.pbio.3002048 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pbio.3002048 | es_ES |
dc.identifier.e-issn | 1545-7885 | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.contributor.funder | Gordon and Betty Moore Foundation | es_ES |
dc.contributor.funder | Biotechnology and Biological Sciences Research Council (UK) | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Volkswagen Foundation | es_ES |
dc.contributor.funder | Merton College | es_ES |
dc.contributor.funder | University of Oxford | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/100000936 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000268 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000769 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100010352 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.pmid | 37014915 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
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systemembra.pdf | 2,7 MB | Adobe PDF | Visualizar/Abrir |
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