Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/338458
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The F-actin cytoskeleton modulates slow secretory components rather than readily releasable vesicle pools in bovine chromaffin cells |
Autor: | Gil, Anabel CSIC; Rueda, Joaquín; Viniegra, Salvador CSIC ORCID; Gutiérrez, Luis M. CSIC ORCID | Fecha de publicación: | 2000 | Editor: | Elsevier | Citación: | Neuroscience 98(3): 605-614 (2000) | Resumen: | Adrenal chromaffin cells were used to test the role of the peripheral cytoskeleton of F-actin in controlling different vesicle pools. Phorbol 12-myristate 13-acetate and calyculin A, two substances affecting phosphorylation–dephosphorylation cycles, produced different degrees of F-actin reorganization, inducing the partial and the almost total disassembly of this structure, respectively, as visualized using rhodamine–phalloidin staining. Consequently, electron microscopy studies revealed the higher efficiency of calyculin-A over phorbol 12-myristate 13-acetate in promoting vesicle access to the plasmalemma boundary. Surprisingly, only the phorbol ester enhanced fast kinetics and the population of rapidly releasable vesicle pools as studied by single-cell amperometry, whereas both agents, as well as the F-actin severing compound, Latrunculin A, promoted an increase in the population of vesicles recruited in response to prolonged or repetitive stimulations. Taken together, our data support the notion that the F-actin peripheral barrier controls primary granule recruitment from reserve vesicle pools, whereas the phorbol ester effect on the rapidly releasable pools might be related to the alteration of late secretory stage through protein kinase C-dependent phosphorylation of an unidentified target. | Versión del editor: | https://doi.org/10.1016/s0306-4522(00)00132-9 | URI: | http://hdl.handle.net/10261/338458 | DOI: | 10.1016/s0306-4522(00)00132-9 | ISSN: | 0306-4522 |
Aparece en las colecciones: | (IN) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
10
checked on 26-abr-2024
SCOPUSTM
Citations
40
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
38
checked on 24-feb-2024
Page view(s)
17
checked on 28-abr-2024
Download(s)
2
checked on 28-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.