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http://hdl.handle.net/10261/330797
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Zhou, Mingqi | es_ES |
dc.contributor.author | Nabilsi, Nancy H. | es_ES |
dc.contributor.author | Wang, Anqi | es_ES |
dc.contributor.author | Gauthier, Marie-Pierre L. | es_ES |
dc.contributor.author | Murray, Kevin O. | es_ES |
dc.contributor.author | Azari, Hassan | es_ES |
dc.contributor.author | Owens, William S. | es_ES |
dc.contributor.author | Newman, Jeremy R. B. | es_ES |
dc.contributor.author | Pardo-Palacios, Francisco J. | es_ES |
dc.contributor.author | Conesa, Ana | es_ES |
dc.contributor.author | Riva, Alberto | es_ES |
dc.contributor.author | Clanton, Thomas L. | es_ES |
dc.contributor.author | Reynolds, Brent A. | es_ES |
dc.contributor.author | Concannon, Patrick | es_ES |
dc.contributor.author | Brant, Jason O. | es_ES |
dc.contributor.author | Bacher, Rhonda | es_ES |
dc.contributor.author | Kladde, Michael P. | es_ES |
dc.date.accessioned | 2023-07-11T07:04:22Z | - |
dc.date.available | 2023-07-11T07:04:22Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | bioRxiv: 515732 (2022) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/330797 | - |
dc.description.abstract | Targeted sequencing is an increasingly sought technology. Available methods, however, are often costly and yield high proportions of off-target reads. Here, we present FENGC, a scalable, multiplexed method in which target sequences are assembled into 5′ flaps for precise excision by flap endonuclease. Recovery of length-matched sequences, amplification with universal primers, and exonucleolytic removal of non-targeted genomic regions mitigate amplification biases and consistently yield ≥80% on-target sequencing. Furthermore, optimized sequential reagent addition and purifications minimize sample loss and facilitate rapid processing of sub-microgram quantities of DNA for detection of genetic variants and DNA methylation. Treatment of cultured human glioblastoma cells and primary murine monocytes with GC methyltransferase followed by FENGC and high-coverage enzymatic methyl sequencing provides single-molecule, long-read detection of differential endogenous CG methylation, dynamic nucleosome repositioning, and transcription factor binding. FENGC provides a versatile and cost-effective platform for targeted sequence enrichment for analysis of genetic and/or epigenetic heterogeneity. | es_ES |
dc.description.sponsorship | This work was supported by grants HDTRA1-16-1-0048 awarded by the Defense Threat Reduction Agency to P.C. and R01 CA155390 awarded by The National Institutes of Health to M.P.K. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Cold Spring Harbor Laboratory Press | es_ES |
dc.relation.isbasedon | The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1101/2022.11.08.515732 | es_ES |
dc.rights | closedAccess | es_ES |
dc.title | Flap-enabled next-generation capture (FENGC): precision targeted single-molecule profiling of epigenetic heterogeneity, chromatin dynamics, and genetic variation | es_ES |
dc.type | preprint | es_ES |
dc.identifier.doi | 10.1101/2022.11.08.515732 | - |
dc.description.peerreviewed | No | es_ES |
dc.relation.publisherversion | https://doi.org/10.1101/2022.11.08.515732 | es_ES |
dc.contributor.funder | National Institutes of Health (US) | es_ES |
dc.contributor.funder | Defense Threat Reduction Agency (US) | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/100000774 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100000002 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_816b | es_ES |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_816b | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | preprint | - |
Aparece en las colecciones: | (I2SysBio) Artículos |
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