Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/329897
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Elucidating the mechanisms of action of antibiotic-like ionic gold and biogenic gold nanoparticles against bacteria |
Autor: | Paesa, Mónica CSIC ORCID; Remirez de Ganuza, Cristina CSIC; Alejo, Teresa CSIC ORCID; Yus, Cristina CSIC ORCID; Irusta, Silvia CSIC ORCID CVN; Arruebo, Manuel CSIC ORCID; Sebastián, Víctor CSIC ORCID; Mendoza, Gracia | Palabras clave: | Ionic gold Gold nanoparticles Antimicrobials Pathogenic bacteria Cellular damage Oxidative stress Proteomics Genomics |
Fecha de publicación: | 2023 | Editor: | Elsevier | Citación: | Journal of Colloid and Interface Science 633: 786-799 (2023) | Resumen: | The antimicrobial action of gold depends on different factors including its oxidation state in the intra- and extracellular medium, the redox potential, its ability to produce reactive oxygen species (ROS), the medium components, the properties of the targeted bacteria wall, its penetration in the bacterial cytosol, the cell membrane potential, and its interaction with intracellular components. We demonstrate that different gold species are able to induce bacterial wall damage as a result of their electrostatic interaction with the cell membrane, the promotion of ROS generation, and the consequent DNA damage. In-depth genomic and proteomic studies on Escherichia coli confirmed the superior toxicity of Au (III) vs Au (I) based on the different molecular mechanisms analyzed including oxidative stress, bacterial energetic metabolism, biosynthetic processes, and cell transport. At equivalent bactericidal doses of Au (III) and Au (I) eukaryotic cells were not as affected as bacteria did, maintaining unaffected cell viability, morphology, and focal adhesions; however, increased ROS generation and disruption in the mitochondrial membrane potential were also observed. Herein, we shed light on the antimicrobial mechanisms of ionic and biogenic gold nanoparticles against bacteria. Under selected conditions antibiotic-like ionic gold can exert a strong antimicrobial activity while being harmless to human cells. | Versión del editor: | https://doi.org/10.1016/j.jcis.2022.11.138 | URI: | http://hdl.handle.net/10261/329897 | DOI: | 10.1016/j.jcis.2022.11.138 | ISSN: | 1095-7103 |
Aparece en las colecciones: | (INMA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
elucidatingbacteria.pdf | 3,23 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
3
checked on 15-may-2024
SCOPUSTM
Citations
12
checked on 19-may-2024
WEB OF SCIENCETM
Citations
7
checked on 23-feb-2024
Page view(s)
34
checked on 22-may-2024
Download(s)
49
checked on 22-may-2024